NMZL miRNA signature
| Gene . | NMZL vs RLN comparison . | NMZL vs FL comparison . | Memory vs GC comparison . | Cytoband . | CNA* . | Putative prediction targets . | |||
|---|---|---|---|---|---|---|---|---|---|
| Fold change . | FDR . | Fold change . | FDR . | Fold change . | P . | ||||
| miR-223 | 0.298 | NS | 0.978 | 0.009 | 1.023 | NS (.054) | Xq12 | — | LMO2, MYBL1 |
| miR-29c | 0.809 | 0.031 | 0.188 | NS | 1.081 | < .001 | 1q32.2 | — | PTEN, PLAG1, GNB4, MEST |
| miR-221 | 0.562 | 0.002 | 0.430 | 0.009 | 1.057 | NS | Xp11.3 | Gain | CD10, LMO2 |
| miR-34a | 0.432 | NS | 0.494 | 0.024 | 0.992 | ND | 1p36.22 | Gain | LEF1, LASS6, GRSF1, E2F3 |
| let-7f | 0.109 | NS | 0.809 | 0.008 | 1.020 | < .05 | 9q22.32 | — | SMAD2, LBR, CCDC100 |
| miR-625 | 0.396 | 0.092 | 0.447 | 0.002 | 1.010 | ND | 14q23.3 | — | PAG1, SFRS1, BAT3, ABCF3 |
| miR-222 | 0.330 | NS | 0.052 | NS | 1.025 | ND | Xp11.3 | Gain | MYO10 |
| miR-202 | 0.320 | NS | −0.036 | NS | 1.045 | ND | 10q26.3 | Gain | MYCBP, LEPROTL1, STX17 |
| miR-765 | −0.207 | NS | −1.491 | 0.000 | 0.990 | ND | 1q23.1 | — | TGF-β1 |
| miR-370 | −0.221 | NS | −1.961 | 0.000 | 0.960 | ND | 14q32.2 | — | TRAF4 |
| miR-513 | −0.230 | NS | −2.226 | 0.000 | 0.939 | ND | Xq27.3 | — | DRAP1, SMARCAD1, HMGB1 |
| miR-494 | −0.937 | NS | −2.969 | 0.000 | 0.989 | < .001 | 14q32.31 | — | CCND2, ASL, PMPCA, BCL6 |
| Gene . | NMZL vs RLN comparison . | NMZL vs FL comparison . | Memory vs GC comparison . | Cytoband . | CNA* . | Putative prediction targets . | |||
|---|---|---|---|---|---|---|---|---|---|
| Fold change . | FDR . | Fold change . | FDR . | Fold change . | P . | ||||
| miR-223 | 0.298 | NS | 0.978 | 0.009 | 1.023 | NS (.054) | Xq12 | — | LMO2, MYBL1 |
| miR-29c | 0.809 | 0.031 | 0.188 | NS | 1.081 | < .001 | 1q32.2 | — | PTEN, PLAG1, GNB4, MEST |
| miR-221 | 0.562 | 0.002 | 0.430 | 0.009 | 1.057 | NS | Xp11.3 | Gain | CD10, LMO2 |
| miR-34a | 0.432 | NS | 0.494 | 0.024 | 0.992 | ND | 1p36.22 | Gain | LEF1, LASS6, GRSF1, E2F3 |
| let-7f | 0.109 | NS | 0.809 | 0.008 | 1.020 | < .05 | 9q22.32 | — | SMAD2, LBR, CCDC100 |
| miR-625 | 0.396 | 0.092 | 0.447 | 0.002 | 1.010 | ND | 14q23.3 | — | PAG1, SFRS1, BAT3, ABCF3 |
| miR-222 | 0.330 | NS | 0.052 | NS | 1.025 | ND | Xp11.3 | Gain | MYO10 |
| miR-202 | 0.320 | NS | −0.036 | NS | 1.045 | ND | 10q26.3 | Gain | MYCBP, LEPROTL1, STX17 |
| miR-765 | −0.207 | NS | −1.491 | 0.000 | 0.990 | ND | 1q23.1 | — | TGF-β1 |
| miR-370 | −0.221 | NS | −1.961 | 0.000 | 0.960 | ND | 14q32.2 | — | TRAF4 |
| miR-513 | −0.230 | NS | −2.226 | 0.000 | 0.939 | ND | Xq27.3 | — | DRAP1, SMARCAD1, HMGB1 |
| miR-494 | −0.937 | NS | −2.969 | 0.000 | 0.989 | < .001 | 14q32.31 | — | CCND2, ASL, PMPCA, BCL6 |
Most relevant miRNAs with significantly differential expression in comparisons of NMZL and RLN, NMZL and FL, and of memory B cells versus GC B cells. Fold changes correspond to the log2 difference between averages. FDR and P values are from the t test (http://pomelo2.bioinfo.cnio.es/). To validate the microarray data, all miRNAs in this table were included in the quantitative RT-PCR assay. Only 5 miRNAs had valuable data in the quantitative RT-PCR assay (Ct value > 36): miR-223, miR-29c, miR-221, let-7f, and miR-494.
NS indicates not significant; —, not applicable; and ND, not determined.
Chromosomal sites with alterations in the CGH microarray data.