Primer sequences used for RT-PCR analysis of XBP1 splicing
| Gene symbol . | Primer sequence (5′-> 3′) . | Amplicon size, bp . |
|---|---|---|
| Xbp1 (h) | Forward: TTGCTGAAGAGGAGGCGGAAG Reverse: GGTCCAAGTTGTCCAGAATGC | Unspliced (210)/spliced (184) |
| Xbp1 (m) | Forward: GAACACGCTTGGGAATGGACAC Reverse: AGAAAGGGAGGCTGGTAAGGAAC | Unspliced (343)/spliced (327) |
| Gene symbol . | Primer sequence (5′-> 3′) . | Amplicon size, bp . |
|---|---|---|
| Xbp1 (h) | Forward: TTGCTGAAGAGGAGGCGGAAG Reverse: GGTCCAAGTTGTCCAGAATGC | Unspliced (210)/spliced (184) |
| Xbp1 (m) | Forward: GAACACGCTTGGGAATGGACAC Reverse: AGAAAGGGAGGCTGGTAAGGAAC | Unspliced (343)/spliced (327) |
h indicates human; and m, mouse.