Table 1 Characteristics and effects of... | American Society of Hematology

Table 1

Characteristics and effects of CD44 antibodies used

Antibody	Isotype	Fine specificity*	Complement binding†	Effect on hyaluronan binding to CD44	Therapeutic in murine autoimmunity	Leukocyte reactive‡	Platelet effects
Antibody	Isotype	Fine specificity*	Complement binding†	Effect on hyaluronan binding to CD44	Therapeutic in murine autoimmunity	Leukocyte reactive‡	Platelet reactive‡	Induce thrombocytopenia‡	Therapeutic in ITP‡
5035-41.1D	Mouse IgG₁	All isoforms	No	—	?	Yes	Yes	NS	Yes
KM114	Rat IgG₁	All isoforms§	No†,‡	↓	?	Yes	Yes	NS	Yes
KM81	Rat IgG_2b	All isoforms§	Yes	↓	Arthritis	Yes	Yes	NS	Yes
KM201	Rat IgG₁	All isoforms§	No	↓	?	Yes	Yes	Yes	Yes
9A4	Rat IgG₁	Variant 6	No	—	?	No	No	NS	No
10D1	Rat IgG₁	Variant 4	No	—	?	No	No	NS	No
IRAWB14	Rat IgG_2a	All isoforms	Yes	↑	?	Yes	Yes	Yes	No
IM7	Rat IgG_2b	All isoforms‖	Yes	—	Arthritis, IDDM, EAE	Yes	Yes	Yes	No

Antibody	Isotype	Fine specificity*	Complement binding†	Effect on hyaluronan binding to CD44	Therapeutic in murine autoimmunity	Leukocyte reactive‡	Platelet effects
Antibody	Isotype	Fine specificity*	Complement binding†	Effect on hyaluronan binding to CD44	Therapeutic in murine autoimmunity	Leukocyte reactive‡	Platelet reactive‡	Induce thrombocytopenia‡	Therapeutic in ITP‡
5035-41.1D	Mouse IgG₁	All isoforms	No	—	?	Yes	Yes	NS	Yes
KM114	Rat IgG₁	All isoforms§	No†,‡	↓	?	Yes	Yes	NS	Yes
KM81	Rat IgG_2b	All isoforms§	Yes	↓	Arthritis	Yes	Yes	NS	Yes
KM201	Rat IgG₁	All isoforms§	No	↓	?	Yes	Yes	Yes	Yes
9A4	Rat IgG₁	Variant 6	No	—	?	No	No	NS	No
10D1	Rat IgG₁	Variant 4	No	—	?	No	No	NS	No
IRAWB14	Rat IgG_2a	All isoforms	Yes	↑	?	Yes	Yes	Yes	No
IM7	Rat IgG_2b	All isoforms‖	Yes	—	Arthritis, IDDM, EAE	Yes	Yes	Yes	No

NS indicates not significant; IDDM, insulin-dependent diabetes mellitus; and EAE, experimental autoimmune encephalitis.

As stated by the manufacturer.

Based on isotype.

Based on the present study at a dose of 50 μg/mouse.

All recognize epitopes in the hyaluronan-binding region.

Recognize a different epitope than KM114 and KM201 (as stated by the antibody data sheets of BD Biosciences PharMingen).