Primers used for qPCR analyses
| Gene . | Forward . | Reverse . |
|---|---|---|
| Annexin A2 | 5′CTCTACACCCCCAAGTGCAT3′ | 5′TCAGTGCTGATGCAAGTTCC3′ |
| E-selectin | 5′TGCGATGCTGCCTACTTGTG3′ | 5′AGAGAGTGCCACTACCAAGGGA3′ |
| TLR4 | 5′AGACCTGTCCCTGAACCCTAT3′ | 5′TTCTAAACCAGCCAGACCTTG3′ |
| ICAM-1 | 5′CGTGGGGAGAAGGAGCTGAA3′ | 5′CAGTGCGGCACGAGAAATTG3′ |
| VCAM-1 | 5′TGGGCTGTGAATCCCCATCT3′ | 5′GGGTCAGCGCGTGGAATTGGTC3′ |
| MyD88 | 5′CTCCTCCACATCCCTTCC3′ | 5′CCGCACGTTCAAGAACAGAGA3′ |
| MD2 | 5′TTCCACCCTGTTTTCTTCCA3′ | 5′AATCGTCATCAGATCCTCGG3′ |
| S100A10 | 5′GAGTTCCCTGGATTTTGGAA3′ | 5′CACTGGTCCAGGTCCTTCAT3′ |
| GAPDH | 5′GCCATCAATGACCCCTTCATT3′ | 5′TCTCGCTCCTGG AAGATGG3′ |
| Gene . | Forward . | Reverse . |
|---|---|---|
| Annexin A2 | 5′CTCTACACCCCCAAGTGCAT3′ | 5′TCAGTGCTGATGCAAGTTCC3′ |
| E-selectin | 5′TGCGATGCTGCCTACTTGTG3′ | 5′AGAGAGTGCCACTACCAAGGGA3′ |
| TLR4 | 5′AGACCTGTCCCTGAACCCTAT3′ | 5′TTCTAAACCAGCCAGACCTTG3′ |
| ICAM-1 | 5′CGTGGGGAGAAGGAGCTGAA3′ | 5′CAGTGCGGCACGAGAAATTG3′ |
| VCAM-1 | 5′TGGGCTGTGAATCCCCATCT3′ | 5′GGGTCAGCGCGTGGAATTGGTC3′ |
| MyD88 | 5′CTCCTCCACATCCCTTCC3′ | 5′CCGCACGTTCAAGAACAGAGA3′ |
| MD2 | 5′TTCCACCCTGTTTTCTTCCA3′ | 5′AATCGTCATCAGATCCTCGG3′ |
| S100A10 | 5′GAGTTCCCTGGATTTTGGAA3′ | 5′CACTGGTCCAGGTCCTTCAT3′ |
| GAPDH | 5′GCCATCAATGACCCCTTCATT3′ | 5′TCTCGCTCCTGG AAGATGG3′ |