Physical, hematological, and serum parameters of 8-week-old Hfe−/− mice harboring 0, 1, or 2 mutant Tmprss6 alleles that are also either null or hemizygous for a liver-specific Hfe transgene
| Genotype . | Body wt, g . | Whole blood . | Serum . | |||||
|---|---|---|---|---|---|---|---|---|
| RBC, × 1012/L . | Hgb, g/L . | Hct . | MCV, × 10−15/L . | MCH, pg . | RDW, % . | Iron, μM . | ||
| Hfe−/−Tmprss6+/+ | 19 ± 1 | 9.2 ± 0.4 | 159 ± 8 | 0.484 ± 0.021 | 52.7 ± 1.1 | 17.3 ± 0.9 | 17.8 ± 1.0 | 41.4 ± 3.4 |
| Hfe−/−Tmprss6+/− | 19 ± 1 | 9.3 ± 0.3 | 158 ± 5 | 0.480 ± 0.019 | 51.5 ± 0.5* | 17.0 ± 0.7 | 17.5 ± 1.0 | 32.3 ± 4.3† |
| Hfe−/−Tmprss6−/− | 18 ± 1* | 11.3 ± 0.4† | 102 ± 8† | 0.327 ± 0.022† | 28.8 ± 1.4† | 9.0 ± 0.5† | 44.1 ± 3.8† | 10.7 ± 2.3† |
| Hfe−/−Tmprss6+/+Hfe tg | 19 ± 2 | 10.3 ± 0.4‡ | 115 ± 3‡ | 0.360 ± 0.011§ | 35.1 ± 1.6‡ | 11.2 ± 0.4‡ | 33.7 ± 2.0‡ | 11.5 ± 2.5‖ |
| Hfe−/−Tmprss6+/−Hfe tg | 19 ± 1 | 10.7 ± 0.6§ | 111 ± 3§¶ | 0.352 ± 0.014§ | 32.9 ± 2.3‡¶ | 10.4 ± 0.5‡# | 36.1 ± 3.1‡ | 11.7 ± 2.5‖ |
| Hfe−/−Tmprss6−/−Hfe tg | 18 ± 2 | 11.1 ± 0.3‖# | 99 ± 5‖# | 0.319 ± 0.012‖# | 28.9 ± 0.7‖# | 9.0 ± 0.2‖# | 44.8 ± 4.1‖# | 9.8 ± 2.0‖ |
| Genotype . | Body wt, g . | Whole blood . | Serum . | |||||
|---|---|---|---|---|---|---|---|---|
| RBC, × 1012/L . | Hgb, g/L . | Hct . | MCV, × 10−15/L . | MCH, pg . | RDW, % . | Iron, μM . | ||
| Hfe−/−Tmprss6+/+ | 19 ± 1 | 9.2 ± 0.4 | 159 ± 8 | 0.484 ± 0.021 | 52.7 ± 1.1 | 17.3 ± 0.9 | 17.8 ± 1.0 | 41.4 ± 3.4 |
| Hfe−/−Tmprss6+/− | 19 ± 1 | 9.3 ± 0.3 | 158 ± 5 | 0.480 ± 0.019 | 51.5 ± 0.5* | 17.0 ± 0.7 | 17.5 ± 1.0 | 32.3 ± 4.3† |
| Hfe−/−Tmprss6−/− | 18 ± 1* | 11.3 ± 0.4† | 102 ± 8† | 0.327 ± 0.022† | 28.8 ± 1.4† | 9.0 ± 0.5† | 44.1 ± 3.8† | 10.7 ± 2.3† |
| Hfe−/−Tmprss6+/+Hfe tg | 19 ± 2 | 10.3 ± 0.4‡ | 115 ± 3‡ | 0.360 ± 0.011§ | 35.1 ± 1.6‡ | 11.2 ± 0.4‡ | 33.7 ± 2.0‡ | 11.5 ± 2.5‖ |
| Hfe−/−Tmprss6+/−Hfe tg | 19 ± 1 | 10.7 ± 0.6§ | 111 ± 3§¶ | 0.352 ± 0.014§ | 32.9 ± 2.3‡¶ | 10.4 ± 0.5‡# | 36.1 ± 3.1‡ | 11.7 ± 2.5‖ |
| Hfe−/−Tmprss6−/−Hfe tg | 18 ± 2 | 11.1 ± 0.3‖# | 99 ± 5‖# | 0.319 ± 0.012‖# | 28.9 ± 0.7‖# | 9.0 ± 0.2‖# | 44.8 ± 4.1‖# | 9.8 ± 2.0‖ |
Complete blood counts and SI were measured in 8-week-old female mice. For each parameter shown, the number of mice per genotype analyzed was as follows, with exceptions shown in parentheses: 8 Hfe−/−Tmprss6+/+, 9 Hfe−/−Tmprss6+/− (8 for SI), 7 Hfe−/−Tmprss6−/− (6 for SI), 8 Hfe−/−Tmprss6+/+Hfe tg (6 for SI), 9 Hfe−/−Tmprss6+/−Hfe tg (8 for SI), and 8 Hfe−/−Tmprss6−/−Hfe tg. Data are presented as means ± SD.
Hfe tg indicates the liver-specific Hfe transgene; Hct, hematocrit; and RDW, red cell distribution width.
P < .05 compared with Hfe−/−Tmprss6+/+ mice.
P < .005 compared with Hfe−/−Tmprss6+/+ mice.
P < .005 compared with Hfe−/−Tmprss6−/− mice.
P < .05 compared with Hfe−/−Tmprss6−/− mice.
P value not significant compared with Hfe−/−Tmprss6−/− mice.
P < .05 compared with Hfe−/−Tmprss6+/+Hfe tg mice.
P < .005 compared with Hfe−/−Tmprss6+/+Hfe tg mice.