Characteristics of AML resulting from genetic interactions between Hoxa9 and wild-type or mutant Pknox1
| Genotype . | AML latency, days . | Spleen weight, mg . | WBC, cells × 109/L . | Blasts, % of total . | GFP+ cells, % BMC . | CD11b+ cells, % BMC . | CD117+ cells, % BMC . | |
|---|---|---|---|---|---|---|---|---|
| PBL . | BM . | |||||||
| Control | NA | 120 ± 13 (20) | 6 ± 1 (5) | NA | 18 ± 3 (1) | NA | 48 (1) | 6 (1) |
| Hoxa9 | 166 ± 14 (8) | 394 ± 77 (4) | 83 ± 41 (6) | 45 ± 6 (6) | 55 ± 6 (5) | NA | ND | ND |
| Hoxa9 + Meis1 | 53 ± 5 (7) | 288 ± 38 (5) | 146 ± 83 (4) | 64 ± 2 (4) | 70 ± 7 (6) | 63 ± 17 (3) | 91 ± 2 (3) | 40 ± 13 (3) |
| Hoxa9 + Pknox1 | 172 ± 8 (12) | 528 ± 51 (9) | 350 (1) | 71 (1) | 60 ± 7 (2) | 50 ± 18 (7) | 91 ± 5 (7) | 17 ± 5 (7) |
| Hoxa9 + Pknox1-ΔC | 155 ± 13 (12) | 547 ± 25 (4) | 193 ± 29 (3) | 39 ± 10 (3) | 60 ± 9 (4) | 78 ± 19 (5) | 91 ± 3 (5) | 34 ± 9 (5) |
| Hoxa9 + Pknox1-SW | 141 ± 11 (18) | 527 ± 53 (10) | 478 ± 195 (6) | 57 ± 6 (6) | 65 ± 5 (6) | 87 ± 5 (6) | 93 ± 2 (6) | 16 ± 5 (6) |
| Hoxa9 + Pknox1-VP16 | 53 ± 5 (15) | 291 ± 21 (15) | 89 ± 30 (13) | 56 ± 4 (13) | 80 ± 5 (11) | 94 ± 2 (5) | 88 ± 2 (5) | 40 ± 4 (4) |
| Hoxa9 + Pknox1-MC | 80 ± 6 (16) | 379 ± 33 (14) | 182 ± 51 (13) | 56 ± 4 (13) | 70 ± 5 (5) | 74 ± 14 (6) | 90 ± 3 (6) | 22 ± 8 (6) |
| Genotype . | AML latency, days . | Spleen weight, mg . | WBC, cells × 109/L . | Blasts, % of total . | GFP+ cells, % BMC . | CD11b+ cells, % BMC . | CD117+ cells, % BMC . | |
|---|---|---|---|---|---|---|---|---|
| PBL . | BM . | |||||||
| Control | NA | 120 ± 13 (20) | 6 ± 1 (5) | NA | 18 ± 3 (1) | NA | 48 (1) | 6 (1) |
| Hoxa9 | 166 ± 14 (8) | 394 ± 77 (4) | 83 ± 41 (6) | 45 ± 6 (6) | 55 ± 6 (5) | NA | ND | ND |
| Hoxa9 + Meis1 | 53 ± 5 (7) | 288 ± 38 (5) | 146 ± 83 (4) | 64 ± 2 (4) | 70 ± 7 (6) | 63 ± 17 (3) | 91 ± 2 (3) | 40 ± 13 (3) |
| Hoxa9 + Pknox1 | 172 ± 8 (12) | 528 ± 51 (9) | 350 (1) | 71 (1) | 60 ± 7 (2) | 50 ± 18 (7) | 91 ± 5 (7) | 17 ± 5 (7) |
| Hoxa9 + Pknox1-ΔC | 155 ± 13 (12) | 547 ± 25 (4) | 193 ± 29 (3) | 39 ± 10 (3) | 60 ± 9 (4) | 78 ± 19 (5) | 91 ± 3 (5) | 34 ± 9 (5) |
| Hoxa9 + Pknox1-SW | 141 ± 11 (18) | 527 ± 53 (10) | 478 ± 195 (6) | 57 ± 6 (6) | 65 ± 5 (6) | 87 ± 5 (6) | 93 ± 2 (6) | 16 ± 5 (6) |
| Hoxa9 + Pknox1-VP16 | 53 ± 5 (15) | 291 ± 21 (15) | 89 ± 30 (13) | 56 ± 4 (13) | 80 ± 5 (11) | 94 ± 2 (5) | 88 ± 2 (5) | 40 ± 4 (4) |
| Hoxa9 + Pknox1-MC | 80 ± 6 (16) | 379 ± 33 (14) | 182 ± 51 (13) | 56 ± 4 (13) | 70 ± 5 (5) | 74 ± 14 (6) | 90 ± 3 (6) | 22 ± 8 (6) |
Values in parentheses are the number of mice analyzed in each category.
NA indicates not applicable; and ND, not done.