Up-regulation of bone-specific markers using quantitative RT-PCR and human specific markers
| . | Naive (2−ΔCt) . | Transplanted (2−ΔCt) . | Fold increase . |
|---|---|---|---|
| Osteocalcin | 4.8 × 10−7 | 5.9 × 10−5 | 123.6 |
| Phosphatase 1 | 4.0 × 10−5 | 9.8 × 10−4 | 24.3 |
| Noggin | 3.1 × 10−4 | 3.1 × 10−2 | 101.1 |
| BMP2 | 3.1 × 10−5 | 3.9 × 10−2 | 128.0 |
| BSP | 2.0 × 10−5 | 4.9 × 10−4 | 24.3 |
| Osteopontin | 1.7 × 10−5 | 5.1 × 10−2 | 29.9 |
| . | Naive (2−ΔCt) . | Transplanted (2−ΔCt) . | Fold increase . |
|---|---|---|---|
| Osteocalcin | 4.8 × 10−7 | 5.9 × 10−5 | 123.6 |
| Phosphatase 1 | 4.0 × 10−5 | 9.8 × 10−4 | 24.3 |
| Noggin | 3.1 × 10−4 | 3.1 × 10−2 | 101.1 |
| BMP2 | 3.1 × 10−5 | 3.9 × 10−2 | 128.0 |
| BSP | 2.0 × 10−5 | 4.9 × 10−4 | 24.3 |
| Osteopontin | 1.7 × 10−5 | 5.1 × 10−2 | 29.9 |
The differences in gene expression reported refer to the expression of the basal levels in undifferentiated human cells. BMP2 indicates bone morphogenetic protein-2; and BSP, bone sialoprotein.