The plating efficiency of expanded cells following ex vivo culture with HDACIs
| Additions to cultures . | BFU-E . | CFU-Mix . | CFU-GM . | Plating efficiency . |
|---|---|---|---|---|
| None* | 27.1 ± 4.5 | 4.6 ± 1.5 | 46.3 ± 12.0 | 16.0 ± 1.6 |
| Cytokines alone | 9.8 ± 2.9 | 2.8 ± 1.6 | 29.6 ± 8.0 | 8.6 ± 1.3 |
| Cytokines + SAHA | 19.2 ± 4.6 | 6.7 ± 2.5 | 42.1 ± 8.1 | 13.1 ± 3.0 |
| Cytokines + VPA | 23.1 ± 3.2 | 8.0 ± 2.5 | 45.8 ± 6.4 | 14.9 ± 2.4† |
| Cytokines + TSA | 18.1 ± 5.9 | 6.9 ± 2.7 | 43.3 ± 5.9 | 13.0 ± 1.7 |
| Additions to cultures . | BFU-E . | CFU-Mix . | CFU-GM . | Plating efficiency . |
|---|---|---|---|---|
| None* | 27.1 ± 4.5 | 4.6 ± 1.5 | 46.3 ± 12.0 | 16.0 ± 1.6 |
| Cytokines alone | 9.8 ± 2.9 | 2.8 ± 1.6 | 29.6 ± 8.0 | 8.6 ± 1.3 |
| Cytokines + SAHA | 19.2 ± 4.6 | 6.7 ± 2.5 | 42.1 ± 8.1 | 13.1 ± 3.0 |
| Cytokines + VPA | 23.1 ± 3.2 | 8.0 ± 2.5 | 45.8 ± 6.4 | 14.9 ± 2.4† |
| Cytokines + TSA | 18.1 ± 5.9 | 6.9 ± 2.7 | 43.3 ± 5.9 | 13.0 ± 1.7 |
Effects of HDACI treatment on plating efficiency of cells following ex vivo culture. CB CD34+ cells (day 0) or the cells cultured either in the presence or absence of HDACIs for 7 days were assayed for HPC.
BFU-E indicates burst-forming unit-erythroid; CFU-Mix, CFU granulocyte, erythroid, monocyte, and megakaryocyte; HPC, hematopoietic progenitor cell; and CFU-GM, CFU–granulocyte macrophage.
Identifies the behavior of the primary CB-CD34+ cells. Five hundred cells were plated in duplicate and each value represents the mean ± SD of 5 independent experiments. The plating efficiency is determined as: (BFU-E + CFU-mix + CFU-GM/number of cells plated) × 100.
The plating efficiency of the cells cultured in the presence of cytokines + VPA was statistically greater than that of the cells cultured with CA (P = 0.003, n = 5).