Blood parameters of WT→WT and βs→WT mice and immunohistochemistry densitometric analysis for murine TF (mTF), murine EPCR (mEPCR), and mAPC in WT→WT and βs→WT mouse brain vessels
| Parameter . | WT→WT . | βs→WT . |
|---|---|---|
| Prothrombin time, s | 10.6 ± 0.5 | 9.9 ± 0.5 |
| Tail bleeding time, s | 316.7 ± 24.4 | 443.3 ± 84.2 |
| Hematocrit level, % | 39.4 ± 1.4 | 32.7 ± 1.8* |
| Total leukocyte count, /μL | 8500.0 ± 365.1 | 14 833.3 ± 823.3* |
| Neutrophil count, /μL | 1583.3 ± 200.7 | 3750.0 ± 359.4* |
| Platelet count, /μL | 1261 ± 132 | 466 ± 31.12* |
| mTF | ||
| Arteriole | 184.3 ± 8.9 | 123.6 ± 3.7* |
| Venule | 69.6 ± 4.2 | 65.3 ± 5.3 |
| mEPCR | ||
| Arteriole | 113.3 ± 1.5 | 92.8 ± 1.6* |
| Venule | 78.2 ± 2.2 | 63.9 ± 2.7 |
| mAPC | ||
| Arteriole | 111.3 ± 2.1 | 62.8 ± 1.6* |
| Venule | 66.1 ± 1.8 | 76.0 ± 1.9 |
| Parameter . | WT→WT . | βs→WT . |
|---|---|---|
| Prothrombin time, s | 10.6 ± 0.5 | 9.9 ± 0.5 |
| Tail bleeding time, s | 316.7 ± 24.4 | 443.3 ± 84.2 |
| Hematocrit level, % | 39.4 ± 1.4 | 32.7 ± 1.8* |
| Total leukocyte count, /μL | 8500.0 ± 365.1 | 14 833.3 ± 823.3* |
| Neutrophil count, /μL | 1583.3 ± 200.7 | 3750.0 ± 359.4* |
| Platelet count, /μL | 1261 ± 132 | 466 ± 31.12* |
| mTF | ||
| Arteriole | 184.3 ± 8.9 | 123.6 ± 3.7* |
| Venule | 69.6 ± 4.2 | 65.3 ± 5.3 |
| mEPCR | ||
| Arteriole | 113.3 ± 1.5 | 92.8 ± 1.6* |
| Venule | 78.2 ± 2.2 | 63.9 ± 2.7 |
| mAPC | ||
| Arteriole | 111.3 ± 2.1 | 62.8 ± 1.6* |
| Venule | 66.1 ± 1.8 | 76.0 ± 1.9 |
Data for hemodynamic parameters of WT→WT and βs→WT mice are expressed as means ± SEM of 5-6 mice/group.
P < .05 compared with WT→WT. Immunohistochemical densitometric analysis for mTF, mPCR, and mAPC was done with AxioVision software. Values (densitometric units) are means ± SEM of 5 tissue sections analyzed from 3 mice per group.