Table 1 Comparison of the culture... | American Society of Hematology

Table 1

Comparison of the culture condition and results in studies on CD34⁺ and CD133⁺ precursors

	Caux et al	Caux et al	Strunk et al	Ito et al	Riedl et al	Mohamadzadeh et al	Mollah et al	Mollah et al	Bartz et al	Hubert et al	Goussetis et al	Current study
Reference	24	24	23	22	18	21	25	25	20	17	19	NA
Year	1992	1992	1996	1999	2000	2001	2003	2003	2003	2005	2006	2010
Seeded cells	Cord blood CD34⁺	Cord blood CD34⁺	Peripheral blood CD34⁺	Peripheral blood CD14⁺	Cord blood CD34⁺	Peripheral blood CD14⁺	Cord blood CD34⁺	Cord blood CD34⁺	Cord blood CD34⁺/CD133⁺	Cord blood CD34⁺	Cord blood CD133⁺	Cord blood CD133⁺
Medium	RPMI 1640	RPMI 1640	IMDM	RPMI 1640	X-VIVO 15	RPMI 1640	X-VIVO 15	X-VIVO 15	RPMI 1640	RPMI 1640	StemSpan	RPMI 1640
Serum	10% FBS	10% FBS	10% FBS	10% human	10% FBS	No	No	10% FBS	10% FBS	10% FBS	No	10% FBS
Cytokines
GM-CSF	+	+	+	+	+	+	+		+	+	+	+
M-CSF								+
SCF	+	+			+		+	+	+	+	+	+
TPO										+	+	+
Flt3L					+		+	+		+	+	+
IL-4			+	+					+	+		+
IL-6											+
IL-15						+
TNF-α	+	+	+		+		+	+	+	+		+
TGF-β1				+	+		+	+	+	+		+
Harvested cells
Dendritic shape	+	+	+	+	+	+	+	+	+	+	+	+
CD1a	+	CD1a⁻/CD14⁺	+	+	+	+	+	+*	+	+	+	+
Birbeck granules	+	−	+/−†	+	Not tested	−	+	+*	−	+	Not tested	−
Langerin	+	−		+	Not tested	+	+	+*	+	+	Not tested	+
Langerin/CD207	Not tested	Not tested	Not tested	+	Not tested	Not tested	Not tested	Not tested	+	+	Not tested	+
Mixed lymphocyte reaction	Potent stimulators	Potent stimulators	Potent stimulators	Potent stimulators	Not tested	Not tested	Potent stimulators	Potent stimulators‡	Potent stimulators§	Not tested	Not tested	Potent stimulators

	Caux et al	Caux et al	Strunk et al	Ito et al	Riedl et al	Mohamadzadeh et al	Mollah et al	Mollah et al	Bartz et al	Hubert et al	Goussetis et al	Current study
Reference	24	24	23	22	18	21	25	25	20	17	19	NA
Year	1992	1992	1996	1999	2000	2001	2003	2003	2003	2005	2006	2010
Seeded cells	Cord blood CD34⁺	Cord blood CD34⁺	Peripheral blood CD34⁺	Peripheral blood CD14⁺	Cord blood CD34⁺	Peripheral blood CD14⁺	Cord blood CD34⁺	Cord blood CD34⁺	Cord blood CD34⁺/CD133⁺	Cord blood CD34⁺	Cord blood CD133⁺	Cord blood CD133⁺
Medium	RPMI 1640	RPMI 1640	IMDM	RPMI 1640	X-VIVO 15	RPMI 1640	X-VIVO 15	X-VIVO 15	RPMI 1640	RPMI 1640	StemSpan	RPMI 1640
Serum	10% FBS	10% FBS	10% FBS	10% human	10% FBS	No	No	10% FBS	10% FBS	10% FBS	No	10% FBS
Cytokines
GM-CSF	+	+	+	+	+	+	+		+	+	+	+
M-CSF								+
SCF	+	+			+		+	+	+	+	+	+
TPO										+	+	+
Flt3L					+		+	+		+	+	+
IL-4			+	+					+	+		+
IL-6											+
IL-15						+
TNF-α	+	+	+		+		+	+	+	+		+
TGF-β1				+	+		+	+	+	+		+
Harvested cells
Dendritic shape	+	+	+	+	+	+	+	+	+	+	+	+
CD1a	+	CD1a⁻/CD14⁺	+	+	+	+	+	+*	+	+	+	+
Birbeck granules	+	−	+/−†	+	Not tested	−	+	+*	−	+	Not tested	−
Langerin	+	−		+	Not tested	+	+	+*	+	+	Not tested	+
Langerin/CD207	Not tested	Not tested	Not tested	+	Not tested	Not tested	Not tested	Not tested	+	+	Not tested	+
Mixed lymphocyte reaction	Potent stimulators	Potent stimulators	Potent stimulators	Potent stimulators	Not tested	Not tested	Potent stimulators	Potent stimulators‡	Potent stimulators§	Not tested	Not tested	Potent stimulators

NA indicates not applicable; FBS, fetal bovine serum; human, human AB serum; M-CSF, macrophage colony-stimulating factor.

More than with GM-CSF.

Langerin (lag)⁺/Birbeck granules (BG)⁺ (Langerhans cells [LC]); lag⁻/BG⁻ (dendritic cells other than LC); lag⁺/BG⁻ (LC-like, minor population).

Higher than with GM-CSF.

In part CLA⁺ and weak stimulators.