Summary statistics: cytokine-stimulated signaling in blast cells
| Cytokine . | pERK . | pS6 . | pSTAT5 . | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Amplitude, normalized MFI . | Peak, minutes . | Interval, minutes . | Amplitude, normalized MFI . | Peak, minutes . | Interval, minutes . | Amplitude, normalized MFI . | Peak, minutes . | Decay, MFI per minute . | |
| NBM | |||||||||
| SCF | 1.00 ± 0.24 | 2.0 ± 0.0 | 9.3 ± 1.4 | 1.00 ± 0.22 | 5.6 ± 0.7 | 40.0 ± 13 | 0.10 ± 0.02 | 2.3 ± 0.4 | ND |
| Flt3L | 0.66 ± 0.20 | 3.4 ± 0.4 | 17.0 ± 2.4 | 0.63 ± 0.25 | 6.0 ± 0.4 | 32.0 ± 5 | 0.10 ± 0.03 | 3.7 ± 1.2 | ND |
| IL3 | 0.06 ± 0.02 | 9.6 ± 1.9 | ND | 0.11 ± 0.03 | 15.0 ± 2.5 | ND | 1.00 ± 0.37 | 12.0 ± 3.2 | −0.013 ± 0.005 |
| GM-CSF | 0.09 ± 0.02 | 3.1 ± 1.0 | ND | 0.30 ± 0.11 | 12.0 ± 7.0 | ND | 0.94 ± 0.29 | 13.0 ± 3.1 | −0.014 ± 0.006 |
| AML1 (M4) | |||||||||
| SCF | 0.53 | 2.0 | 20.0* | 3.30 | 10.0* | 60.0* | 0.13 | 2.0 | ND |
| Flt3L | 0.27 | 4.0 | 20.0 | 0.93 | 10.0* | 60.0* | 0.03 | 2.0 | ND |
| IL3 | 0.02 | 6.0 | ND | 0.10 | 0.0 | ND | 0.27 | 6.0 | −0.004 |
| GM-CSF | 0.04 | 6.0 | ND | 0.07 | 0.0 | ND | 0.41 | 6.0 | −0.007 |
| AML2 (M2) | |||||||||
| SCF | 0.58 | 2 | 20.0* | 3.30* | 10.0* | 30.0 | 0.1 | 2.0 | ND |
| AML3 (M4Eo) | |||||||||
| SCF | 2.10* | 4.0* | 60.0* | 5.30* | 10.0* | 60.0* | 0.23 | 2.0 | ND |
| Flt3L | 2.10* | 6.0* | 60.0* | 4.60* | 10.0* | 60.0* | 0.21 | 6.0 | ND |
| AML5 (M1) | |||||||||
| SCF | 0.08 | 0.48 | |||||||
| Flt3L | 1.90* | 4.0 | 60.0* | 7.60* | 6.0 | 60.0* | ND | ND | ND |
| Cytokine . | pERK . | pS6 . | pSTAT5 . | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Amplitude, normalized MFI . | Peak, minutes . | Interval, minutes . | Amplitude, normalized MFI . | Peak, minutes . | Interval, minutes . | Amplitude, normalized MFI . | Peak, minutes . | Decay, MFI per minute . | |
| NBM | |||||||||
| SCF | 1.00 ± 0.24 | 2.0 ± 0.0 | 9.3 ± 1.4 | 1.00 ± 0.22 | 5.6 ± 0.7 | 40.0 ± 13 | 0.10 ± 0.02 | 2.3 ± 0.4 | ND |
| Flt3L | 0.66 ± 0.20 | 3.4 ± 0.4 | 17.0 ± 2.4 | 0.63 ± 0.25 | 6.0 ± 0.4 | 32.0 ± 5 | 0.10 ± 0.03 | 3.7 ± 1.2 | ND |
| IL3 | 0.06 ± 0.02 | 9.6 ± 1.9 | ND | 0.11 ± 0.03 | 15.0 ± 2.5 | ND | 1.00 ± 0.37 | 12.0 ± 3.2 | −0.013 ± 0.005 |
| GM-CSF | 0.09 ± 0.02 | 3.1 ± 1.0 | ND | 0.30 ± 0.11 | 12.0 ± 7.0 | ND | 0.94 ± 0.29 | 13.0 ± 3.1 | −0.014 ± 0.006 |
| AML1 (M4) | |||||||||
| SCF | 0.53 | 2.0 | 20.0* | 3.30 | 10.0* | 60.0* | 0.13 | 2.0 | ND |
| Flt3L | 0.27 | 4.0 | 20.0 | 0.93 | 10.0* | 60.0* | 0.03 | 2.0 | ND |
| IL3 | 0.02 | 6.0 | ND | 0.10 | 0.0 | ND | 0.27 | 6.0 | −0.004 |
| GM-CSF | 0.04 | 6.0 | ND | 0.07 | 0.0 | ND | 0.41 | 6.0 | −0.007 |
| AML2 (M2) | |||||||||
| SCF | 0.58 | 2 | 20.0* | 3.30* | 10.0* | 30.0 | 0.1 | 2.0 | ND |
| AML3 (M4Eo) | |||||||||
| SCF | 2.10* | 4.0* | 60.0* | 5.30* | 10.0* | 60.0* | 0.23 | 2.0 | ND |
| Flt3L | 2.10* | 6.0* | 60.0* | 4.60* | 10.0* | 60.0* | 0.21 | 6.0 | ND |
| AML5 (M1) | |||||||||
| SCF | 0.08 | 0.48 | |||||||
| Flt3L | 1.90* | 4.0 | 60.0* | 7.60* | 6.0 | 60.0* | ND | ND | ND |
Data are mean ± SEM. Amplitude is defined as the maximum cytokine-stimulated response; amplitude data are normalized to the maximum response of each phosphoprotein in NBM. Peak is the time to maximum response. Interval is the duration of response from rise through decay, where normalized MFI ≥ 0.05. Decay is the slope from 15 to 30 minutes for the IL-3 and GM-CSF → pStat5 signal.
ND indicates not determined, generally because the data were either noisy or multimodal.
P < .05 compared with the corresponding value in NBM.