Primers used for ChiP and RNA analysis
| Gene . | Species . | Forward sequence . | Reverse sequence . |
|---|---|---|---|
| ChIP | |||
| NFKB1 proximal | Human | GACGTCAGTGGGAATTTCC | GTGGCGAAACCTCCTCTTC |
| NFKB1 upstream | Human | TGCTGCATGGAATTACATCC | CATCTTAACTTGATAACATTTGC |
| Nfkb1 proximal 1 | Mouse | GTCCGTCCGTCTGTCTGCTC | CTCCTCTTCCTGCCCGGC |
| Nfkb1 proximal 2 | Mouse | CTCGACGTCAGTGGGAATTT | GCGAAACCTCCTCTTCCTG |
| Nfkb1 upstream | Mouse | CTTCATAAGTGGAACGCTGAG | GGTCTCACTACTGAGTTCAAG |
| Flip proximal 1 | Mouse | ATGGCCTCGATTCAGTTTTG | GATAAGGACGTCCCCAGAGT |
| Flip proximal 2 | Mouse | CTGTAACTTTCCTCCGCCCT | GAGAAGGGCTCCGAGAAGAT |
| Flip upstream | Mouse | CAAGGAGGTCGCTACGAGTC | AGCCAGCAAAGCCAGTAAAG |
| Reverse transcriptase PCR | |||
| Nfkb1 | Mouse | GGTATGCACCGTAACAGCAG | GCTTCCCTCTGTCATCCGT |
| RelA | Mouse | CTCATCCACATGAACTTGTGG | GCTTCTTCACACACTGGATC |
| mS16 | Mouse | CTTGGAGGCTTCATCCACAT | ATATTCGGGTCCGTGTGAAG |
| NFKB1 | Human | CTGTGGTTGAAATACTTCTGG | CAGAATGGCAGAAGATGATC |
| FLIP | Human | GGATAAATCTGATGTGTCCTC | ACTCAACCACAAGGTCCAAG |
| CEBPA | Human | AAGAAGTCGGTGGACAAGAACAG | GCGGTCATTGTCACTGGTCA |
| ABL | Human | AACACCCTAACCTGGTGCAG | CAAGTGGTTCTCCCCTACCA |
| GAPDH | Human | CCACCCATGGCAAATTCC | GATGGGATTTCCATTGATGACA |
| Actin | Human | TGCGTGACATTAAGGAGAAG | GCTCGTAGCTCTTCTCCA |
| Gene . | Species . | Forward sequence . | Reverse sequence . |
|---|---|---|---|
| ChIP | |||
| NFKB1 proximal | Human | GACGTCAGTGGGAATTTCC | GTGGCGAAACCTCCTCTTC |
| NFKB1 upstream | Human | TGCTGCATGGAATTACATCC | CATCTTAACTTGATAACATTTGC |
| Nfkb1 proximal 1 | Mouse | GTCCGTCCGTCTGTCTGCTC | CTCCTCTTCCTGCCCGGC |
| Nfkb1 proximal 2 | Mouse | CTCGACGTCAGTGGGAATTT | GCGAAACCTCCTCTTCCTG |
| Nfkb1 upstream | Mouse | CTTCATAAGTGGAACGCTGAG | GGTCTCACTACTGAGTTCAAG |
| Flip proximal 1 | Mouse | ATGGCCTCGATTCAGTTTTG | GATAAGGACGTCCCCAGAGT |
| Flip proximal 2 | Mouse | CTGTAACTTTCCTCCGCCCT | GAGAAGGGCTCCGAGAAGAT |
| Flip upstream | Mouse | CAAGGAGGTCGCTACGAGTC | AGCCAGCAAAGCCAGTAAAG |
| Reverse transcriptase PCR | |||
| Nfkb1 | Mouse | GGTATGCACCGTAACAGCAG | GCTTCCCTCTGTCATCCGT |
| RelA | Mouse | CTCATCCACATGAACTTGTGG | GCTTCTTCACACACTGGATC |
| mS16 | Mouse | CTTGGAGGCTTCATCCACAT | ATATTCGGGTCCGTGTGAAG |
| NFKB1 | Human | CTGTGGTTGAAATACTTCTGG | CAGAATGGCAGAAGATGATC |
| FLIP | Human | GGATAAATCTGATGTGTCCTC | ACTCAACCACAAGGTCCAAG |
| CEBPA | Human | AAGAAGTCGGTGGACAAGAACAG | GCGGTCATTGTCACTGGTCA |
| ABL | Human | AACACCCTAACCTGGTGCAG | CAAGTGGTTCTCCCCTACCA |
| GAPDH | Human | CCACCCATGGCAAATTCC | GATGGGATTTCCATTGATGACA |
| Actin | Human | TGCGTGACATTAAGGAGAAG | GCTCGTAGCTCTTCTCCA |