MCPyV detection in the DNA of isolated CLL leukemic cells
| ID . | Sex . | Age . | β-glo. . | LT3 . | M1/M2 . | VP1 . | MCPyV . | LTAgdel . | wt+del . | Stage* . | CD38 . | ZAP70 . | Thym.kin. . | LCDT . | Cytogenetics . | IgVH . |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CLL 1 | M | 77 | + | - | - | - | - | C | + | + | unmutated | |||||
| CLL 2 | M | 77 | + | - | + | - | + | + | A | - | - | - | del13q14 | mutated | ||
| CLL 3 | F | uk | + | - | - | - | - | |||||||||
| CLL 4 | M | 70 | + | + | - | - | + | + | + | A | - | - | - | + | normal | unmutated |
| CLL 5 | F | 77 | + | - | - | - | - | A | - | - | - | - | del13q14 | mutated | ||
| CLL 6 | M | 64 | + | - | - | - | - | B | + | + | + | + | del11 | unmutated | ||
| CLL 7 | M | 81 | + | - | + | - | + | + | + | A | - | - | - | - | del13q14 homozyg. | unmutated |
| CLL 8 | M | 66 | + | - | - | - | - | B | - | - | - | - | del13q14 | |||
| CLL 9 | M | 102 | + | - | - | + | + | + | - | - | - | normal | ||||
| CLL 10 | M | 27 | + | - | + | - | + | B | + | + | + | + | 11q | |||
| CLL 11 | F | 78 | + | - | + | - | + | + | - | - | + | |||||
| CLL 12 | M | 63 | + | - | - | - | - | B | - | - | - | - | normal | |||
| CLL 13 | M | 65 | + | - | - | - | - | C | - | - | + | + | del17p | unmutated | ||
| CLL 14 | M | 67 | + | - | - | - | - | B | + | + | + | + | tris12 | mutated | ||
| CLL 15 | F | 61 | + | - | - | - | - | A | - | + | - | - | del13q14 | |||
| CLL 16 | M | 60 | + | - | - | - | - | C | + | - | + | tris12, del13q14 | mutated | |||
| CLL 17 | M | 59 | + | + | + | - | + | + | B | - | + | + | + | normal | ||
| CLL 18 | M | 71 | + | - | - | - | - | C | + | + | + | + | unmutated | |||
| CLL 19 | M | 85 | + | - | - | - | - | - | - | mutated | ||||||
| CLL 20 | M | 76 | + | - | - | - | - | C | - | + | + | + | ||||
| CLL 21 | F | 62 | + | - | + | - | + | C | - | - | normal | |||||
| CLL 22 | M | 53 | + | - | + | - | + | B | + | - | + | - | mutated | |||
| CLL 23 | M | 81 | + | - | + | - | + | B | + | |||||||
| CLL 24 | M | 67 | + | - | + | - | + | A | - | - | + | mutated | ||||
| CLL 25 | M | 68 | + | - | + | - | + | C | ||||||||
| CLL 26 | M | 63 | + | - | - | - | - | C | - | + | 13q, 11q | |||||
| CLL 27 | M | 67 | + | - | + | - | + | A | - | + | + | - | mutated | |||
| CLL 28 | M | 59 | + | - | - | - | - | C | + | |||||||
| CLL 29 | F | 76 | + | - | - | - | - | A | - | + | - | del13q14 homozyg. | ||||
| CLL 30 | M | 67 | + | - | - | - | - | C | + | + | + | |||||
| CLL 31 | F | 70 | + | - | - | - | - | C | - | - | + | + | 13q, 17p, transloc. | unmutated | ||
| CLL 32 | F | 60 | + | - | - | - | - | B | ||||||||
| CLL 34 | F | 66 | + | - | - | - | - | A | - | - | - | - | ||||
| CLL 35 | F | 62 | + | - | + | - | + | |||||||||
| CLL 36 | F | 56 | + | - | - | - | - | A | - | - | - | mutated | ||||
| CLL 37 | M | 60 | + | - | - | - | - | A | - | + | - | |||||
| CLL 38 | M | 51 | + | - | - | - | - | A | - | + | + | normal | mutated | |||
| CLL 39 | M | 62 | + | - | - | - | - | A | - | + | - | - | ||||
| CLL 40 | F | 90 | + | - | - | - | - | A | - | - | - | - | ||||
| CLL 41 | F | 81 | + | - | - | - | - | A | - | - | - | del13q14 | mutated | |||
| CLL 42 | M | 56 | + | - | - | - | - | C | + | - | + | + | unmutated | |||
| CLL 43 | M | 68 | + | - | + | - | + | A | - | + | - | - | del13q14 homozyg. | mutated | ||
| CLL 44 | F | 79 | + | - | - | - | - | B | - | del13q14 | unmutated | |||||
| CLL 45 | M | 79 | + | - | + | - | + | C | + | + | + | + | normal | mutated | ||
| CLL 46 | M | 68 | + | - | + | - | + | + | - | - | normal | unmutated | ||||
| CLL 47 | M | 61 | + | - | - | - | - | A | - | + | + | del13q14 | mutated | |||
| CLL 48 | F | 77 | + | - | - | - | - | + | - | + | del13q14 homozyg. | unmutated | ||||
| CLL 49 | F | 57 | + | - | - | - | - | - | - | - | - | del13q14 | mutated | |||
| CLL 50 | M | 55 | + | - | + | - | + | - | + | - | normal | |||||
| CLL 51 | F | 71 | + | - | - | - | - | + | + | - | normal | |||||
| CLL 53 | F | 68 | + | - | - | - | - | + | + | + | normal | unmutated | ||||
| CLL 54 | F | 78 | + | - | - | - | - | A | - | + | - | del13q14 | ||||
| CLL 55 | F | 45 | + | - | - | - | - | A | - | - | - | + | normal | |||
| CLL 56 | M | 65 | + | - | - | - | - | C | + | + | ||||||
| CLL 57 | M | 76 | + | - | - | - | - | - | - | - | del13q14 homozyg. | |||||
| CLL 58 | M | 68 | + | - | - | - | - | - | - | - | del13q14 | |||||
| CLL 59 | M | 61 | + | - | - | - | - | C | - | + | ||||||
| CLL 61 | M | 46 | + | - | - | - | - | A | - | - | + | - | del13q14 homozyg. | mutated | ||
| CLL 62 | M | 68 | + | - | - | - | - | C | - | + | + | - | tri12, del11q | |||
| CLL 63 | F | 72 | + | - | - | - | - | A | - | - | ||||||
| CLL 64 | M | 61 | + | - | - | - | - | A | - | - | - | - | ||||
| CLL 66 | M | 51 | + | - | - | - | - | + | ||||||||
| CLL 67 | M | 75 | + | - | - | - | - | C | - | - | + | - | ||||
| CLL 68 | F | 54 | + | - | - | - | - | A | - | - | + | + | del13q14 | mutated | ||
| CLL 69 | M | 66 | + | - | - | - | - | A | ||||||||
| CLL 70 | M | 48 | + | - | - | - | - | A | - | - | ||||||
| CLL 71 | M | 45 | + | - | - | - | - | A | - | normal | ||||||
| CLL 73 | M | 48 | + | - | - | + | + | B | + | - | - | - | 13q, 6q, transloc. | unmutated | ||
| CLL 74 | F | 57 | + | - | - | - | - | A | - | - | - | - | normal | mutated | ||
| CLL 75 | M | 83 | + | - | - | - | - | B | - | + | - | - |
| ID . | Sex . | Age . | β-glo. . | LT3 . | M1/M2 . | VP1 . | MCPyV . | LTAgdel . | wt+del . | Stage* . | CD38 . | ZAP70 . | Thym.kin. . | LCDT . | Cytogenetics . | IgVH . |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CLL 1 | M | 77 | + | - | - | - | - | C | + | + | unmutated | |||||
| CLL 2 | M | 77 | + | - | + | - | + | + | A | - | - | - | del13q14 | mutated | ||
| CLL 3 | F | uk | + | - | - | - | - | |||||||||
| CLL 4 | M | 70 | + | + | - | - | + | + | + | A | - | - | - | + | normal | unmutated |
| CLL 5 | F | 77 | + | - | - | - | - | A | - | - | - | - | del13q14 | mutated | ||
| CLL 6 | M | 64 | + | - | - | - | - | B | + | + | + | + | del11 | unmutated | ||
| CLL 7 | M | 81 | + | - | + | - | + | + | + | A | - | - | - | - | del13q14 homozyg. | unmutated |
| CLL 8 | M | 66 | + | - | - | - | - | B | - | - | - | - | del13q14 | |||
| CLL 9 | M | 102 | + | - | - | + | + | + | - | - | - | normal | ||||
| CLL 10 | M | 27 | + | - | + | - | + | B | + | + | + | + | 11q | |||
| CLL 11 | F | 78 | + | - | + | - | + | + | - | - | + | |||||
| CLL 12 | M | 63 | + | - | - | - | - | B | - | - | - | - | normal | |||
| CLL 13 | M | 65 | + | - | - | - | - | C | - | - | + | + | del17p | unmutated | ||
| CLL 14 | M | 67 | + | - | - | - | - | B | + | + | + | + | tris12 | mutated | ||
| CLL 15 | F | 61 | + | - | - | - | - | A | - | + | - | - | del13q14 | |||
| CLL 16 | M | 60 | + | - | - | - | - | C | + | - | + | tris12, del13q14 | mutated | |||
| CLL 17 | M | 59 | + | + | + | - | + | + | B | - | + | + | + | normal | ||
| CLL 18 | M | 71 | + | - | - | - | - | C | + | + | + | + | unmutated | |||
| CLL 19 | M | 85 | + | - | - | - | - | - | - | mutated | ||||||
| CLL 20 | M | 76 | + | - | - | - | - | C | - | + | + | + | ||||
| CLL 21 | F | 62 | + | - | + | - | + | C | - | - | normal | |||||
| CLL 22 | M | 53 | + | - | + | - | + | B | + | - | + | - | mutated | |||
| CLL 23 | M | 81 | + | - | + | - | + | B | + | |||||||
| CLL 24 | M | 67 | + | - | + | - | + | A | - | - | + | mutated | ||||
| CLL 25 | M | 68 | + | - | + | - | + | C | ||||||||
| CLL 26 | M | 63 | + | - | - | - | - | C | - | + | 13q, 11q | |||||
| CLL 27 | M | 67 | + | - | + | - | + | A | - | + | + | - | mutated | |||
| CLL 28 | M | 59 | + | - | - | - | - | C | + | |||||||
| CLL 29 | F | 76 | + | - | - | - | - | A | - | + | - | del13q14 homozyg. | ||||
| CLL 30 | M | 67 | + | - | - | - | - | C | + | + | + | |||||
| CLL 31 | F | 70 | + | - | - | - | - | C | - | - | + | + | 13q, 17p, transloc. | unmutated | ||
| CLL 32 | F | 60 | + | - | - | - | - | B | ||||||||
| CLL 34 | F | 66 | + | - | - | - | - | A | - | - | - | - | ||||
| CLL 35 | F | 62 | + | - | + | - | + | |||||||||
| CLL 36 | F | 56 | + | - | - | - | - | A | - | - | - | mutated | ||||
| CLL 37 | M | 60 | + | - | - | - | - | A | - | + | - | |||||
| CLL 38 | M | 51 | + | - | - | - | - | A | - | + | + | normal | mutated | |||
| CLL 39 | M | 62 | + | - | - | - | - | A | - | + | - | - | ||||
| CLL 40 | F | 90 | + | - | - | - | - | A | - | - | - | - | ||||
| CLL 41 | F | 81 | + | - | - | - | - | A | - | - | - | del13q14 | mutated | |||
| CLL 42 | M | 56 | + | - | - | - | - | C | + | - | + | + | unmutated | |||
| CLL 43 | M | 68 | + | - | + | - | + | A | - | + | - | - | del13q14 homozyg. | mutated | ||
| CLL 44 | F | 79 | + | - | - | - | - | B | - | del13q14 | unmutated | |||||
| CLL 45 | M | 79 | + | - | + | - | + | C | + | + | + | + | normal | mutated | ||
| CLL 46 | M | 68 | + | - | + | - | + | + | - | - | normal | unmutated | ||||
| CLL 47 | M | 61 | + | - | - | - | - | A | - | + | + | del13q14 | mutated | |||
| CLL 48 | F | 77 | + | - | - | - | - | + | - | + | del13q14 homozyg. | unmutated | ||||
| CLL 49 | F | 57 | + | - | - | - | - | - | - | - | - | del13q14 | mutated | |||
| CLL 50 | M | 55 | + | - | + | - | + | - | + | - | normal | |||||
| CLL 51 | F | 71 | + | - | - | - | - | + | + | - | normal | |||||
| CLL 53 | F | 68 | + | - | - | - | - | + | + | + | normal | unmutated | ||||
| CLL 54 | F | 78 | + | - | - | - | - | A | - | + | - | del13q14 | ||||
| CLL 55 | F | 45 | + | - | - | - | - | A | - | - | - | + | normal | |||
| CLL 56 | M | 65 | + | - | - | - | - | C | + | + | ||||||
| CLL 57 | M | 76 | + | - | - | - | - | - | - | - | del13q14 homozyg. | |||||
| CLL 58 | M | 68 | + | - | - | - | - | - | - | - | del13q14 | |||||
| CLL 59 | M | 61 | + | - | - | - | - | C | - | + | ||||||
| CLL 61 | M | 46 | + | - | - | - | - | A | - | - | + | - | del13q14 homozyg. | mutated | ||
| CLL 62 | M | 68 | + | - | - | - | - | C | - | + | + | - | tri12, del11q | |||
| CLL 63 | F | 72 | + | - | - | - | - | A | - | - | ||||||
| CLL 64 | M | 61 | + | - | - | - | - | A | - | - | - | - | ||||
| CLL 66 | M | 51 | + | - | - | - | - | + | ||||||||
| CLL 67 | M | 75 | + | - | - | - | - | C | - | - | + | - | ||||
| CLL 68 | F | 54 | + | - | - | - | - | A | - | - | + | + | del13q14 | mutated | ||
| CLL 69 | M | 66 | + | - | - | - | - | A | ||||||||
| CLL 70 | M | 48 | + | - | - | - | - | A | - | - | ||||||
| CLL 71 | M | 45 | + | - | - | - | - | A | - | normal | ||||||
| CLL 73 | M | 48 | + | - | - | + | + | B | + | - | - | - | 13q, 6q, transloc. | unmutated | ||
| CLL 74 | F | 57 | + | - | - | - | - | A | - | - | - | - | normal | mutated | ||
| CLL 75 | M | 83 | + | - | - | - | - | B | - | + | - | - |
ID indicates patient identification; M, male; F, female; β-glo., β-globin PCR; LT3, M1/M2, VP1, names of the primers used for MCPyV detection according to Feng et al1 ; MCPyV, Merkel cell polyomavirus; LTAg del, MCPyV harboring the 246bp deletion; stage*, according to Binet; thym.kin., thymidine kinase; LCDT, lymphocyte doubling time; IgVH, IgVH hypermutational status; and uk, unknown.