Detection of enriched histone H3 acetylation islands in the 3′ region of the Notch1 gene in N1C+ thymocytes
| . | Island 1 (I1) . | Island 2 (I2) . |
|---|---|---|
| Coordinates (chromosome 2) | 26320200-26328599 | 26313600-26319199 |
| Island score (range, 30-15 000) | 245 | 138 |
| Input tag number | 66 | 46 |
| WT tag number | 80 | 83 |
| N1C+ tag number | 231 | 145 |
| P (vs input) | 9.8 × 10−57 | 3.7 × 10−31 |
| P (vs wild-type) | 9.9 × 10−43 | 1.6 × 10−11 |
| . | Island 1 (I1) . | Island 2 (I2) . |
|---|---|---|
| Coordinates (chromosome 2) | 26320200-26328599 | 26313600-26319199 |
| Island score (range, 30-15 000) | 245 | 138 |
| Input tag number | 66 | 46 |
| WT tag number | 80 | 83 |
| N1C+ tag number | 231 | 145 |
| P (vs input) | 9.8 × 10−57 | 3.7 × 10−31 |
| P (vs wild-type) | 9.9 × 10−43 | 1.6 × 10−11 |
Chromatin immunoprecipitation sequence data were analyzed with the “statistical model for identification of chip-enriched regions” algorithm to detect islands of enriched H3ac. Two islands (I1 and I2, see Figure 6A for positions) were predicted in the Notch1 gene in N1C+ cells but not in wild-type thymocytes or input. Tag numbers were calculated by multiplying the raw data with a correction factor that corresponds to the total sequence tags in the N1C+ sample divided by the combined total sequenced tags in all samples (9.3 × 106, 14.8 × 106, and 12.8 × 106 for input, wild-type, and N1C+, respectively). The coordinates were obtained from the Mm9 build of the mouse genome.