Phenotype of immature DCs, DC-10, and mature DCs
| . | iDCs . | DC-10 . | mDCs . | |||
|---|---|---|---|---|---|---|
| Mean ± SD . | n . | Mean ± SD . | n . | Mean ± SD . | n . | |
| CD14 | 3 ± 8 | 36 | 78 ± 14 | 36 | 1 ± 3 | 36 |
| CD16 | 15 ± 17 | 15 | 81 ± 10 | 15 | 1 ± 1 | 15 |
| CD11c | 88 ± 20 | 25 | 88 ± 18 | 25 | 88 ± 17 | 25 |
| CD11b | 93 ± 11 | 25 | 93 ± 4 | 25 | 92 ± 8 | 25 |
| CD1a | 67 ± 24 | 34 | 3 ± 6 | 34 | 64 ± 19 | 34 |
| CD83 | 18 ± 25 | 32 | 52 ± 30 | 32 | 60 ± 22 | 32 |
| CD80 | 19 ± 18 | 20 | 62 ± 29 | 20 | 62 ± 34 | 20 |
| CD86 | 34 ± 30 | 22 | 63 ± 38 | 22 | 94 ± 12 | 22 |
| HLA-DR | 52 ± 28 | 29 | 85 ± 18 | 29 | 84 ± 26 | 29 |
| . | iDCs . | DC-10 . | mDCs . | |||
|---|---|---|---|---|---|---|
| Mean ± SD . | n . | Mean ± SD . | n . | Mean ± SD . | n . | |
| CD14 | 3 ± 8 | 36 | 78 ± 14 | 36 | 1 ± 3 | 36 |
| CD16 | 15 ± 17 | 15 | 81 ± 10 | 15 | 1 ± 1 | 15 |
| CD11c | 88 ± 20 | 25 | 88 ± 18 | 25 | 88 ± 17 | 25 |
| CD11b | 93 ± 11 | 25 | 93 ± 4 | 25 | 92 ± 8 | 25 |
| CD1a | 67 ± 24 | 34 | 3 ± 6 | 34 | 64 ± 19 | 34 |
| CD83 | 18 ± 25 | 32 | 52 ± 30 | 32 | 60 ± 22 | 32 |
| CD80 | 19 ± 18 | 20 | 62 ± 29 | 20 | 62 ± 34 | 20 |
| CD86 | 34 ± 30 | 22 | 63 ± 38 | 22 | 94 ± 12 | 22 |
| HLA-DR | 52 ± 28 | 29 | 85 ± 18 | 29 | 84 ± 26 | 29 |
Monocyte-derived DCs were differentiated in IL-4 and GM-CSF in the presence of IL-10 for 7 days (DC-10), or in IL-4 and GM-CSF for 7 days (iDCs), or in IL-4 and GM-CSF for 5 days and cultured for additional 2 days with LPS (mDCs). Expression of CD14, CD16, CD11c, CD11b, CD1a, CD83, CD80, CD86, and HLA-DR was evaluated by FACS analysis.
n indicates the number of samples analyzed and derived from different healthy donors.