A2t-induced cytokine production by WT and TLR4-deficient BMDM
| . | WT, A2 . | TLR4−/−, A2t . | WT, LPS . | TLR4−/−, LPS . |
|---|---|---|---|---|
| IL-1α | 12 | <LL | 43 | <LL |
| IL-6 | 302 | <LL | 6012 | <LL |
| TNF-α | 1154 | 12 | 1312 | 9 |
| MCP1 | 653 | 78 | 1823 | 180 |
| MIP-1α | 84 | 50 | 260 | 50 |
| IP10 | 18 735 | 693 | 17 826 | 402 |
| RANTES | 805 | 12 | 1763 | 6 |
| . | WT, A2 . | TLR4−/−, A2t . | WT, LPS . | TLR4−/−, LPS . |
|---|---|---|---|---|
| IL-1α | 12 | <LL | 43 | <LL |
| IL-6 | 302 | <LL | 6012 | <LL |
| TNF-α | 1154 | 12 | 1312 | 9 |
| MCP1 | 653 | 78 | 1823 | 180 |
| MIP-1α | 84 | 50 | 260 | 50 |
| IP10 | 18 735 | 693 | 17 826 | 402 |
| RANTES | 805 | 12 | 1763 | 6 |
WT or TLR-deficient (TLR4−/−) 7-day-old murine BMDM were treated with 5nM A2t or 10 ng/mL LPS for 24 hours, and levels of the indicated cytokines (in pg/mL) were measured in the supernatants by luminex. Macrophages were pooled from 5 females per genotype; supernatants were assayed in duplicate.
LL indicates lower limit of quantitation; other abbreviations as in Table 1.