Table 1

VH gene analysis of murine gastric MALT lymphoma cases

CaseVH segmentHomology %Clones sequencedSomatic* mutationFR/CDRRSAntigen selectionPIntraclonal variationCDR3 length
mu-1 IGHV1-S130*01 93.06 11 FR 42 − .490 11 
     CDR 29 .000   
mu-2 IGHV14-3*02 92.36 10 FR 13 − .391 15 
     CDR .059   
mu-3 IGHV1-S81*02 97.92 13 FR .562 − 13 
     CDR − .801   
mu-4 IGHV14-3*02 99.31 10 − FR .000 13 
     CDR − .621   
mu-5 IGHV1-69*02 86.46 FR 24 − .491 − 10 
     CDR − .601   
mu-6 IGHV1-67*1 80.90 10 FR .03 − 12 
     CDR − .110   
mu-7 IGHV1-S22*01 94.62 22 FR − .112 − 15 
     CDR − .009   
mu-8 IGHV14-3*02 98.61 − FR .329 11 
     CDR − 1.278   
mu-9 IGHV1-18*02 88.19 FR 18 10 .107 12 
     CDR .369   
mu-10 IGHV1-S81*02 95.83 10 FR − .23 14 
     CDR − .156   
mu-11 IGHV1-74*01 86.81 10 FR 17 .12 − 12 
     CDR 10 .034   
CaseVH segmentHomology %Clones sequencedSomatic* mutationFR/CDRRSAntigen selectionPIntraclonal variationCDR3 length
mu-1 IGHV1-S130*01 93.06 11 FR 42 − .490 11 
     CDR 29 .000   
mu-2 IGHV14-3*02 92.36 10 FR 13 − .391 15 
     CDR .059   
mu-3 IGHV1-S81*02 97.92 13 FR .562 − 13 
     CDR − .801   
mu-4 IGHV14-3*02 99.31 10 − FR .000 13 
     CDR − .621   
mu-5 IGHV1-69*02 86.46 FR 24 − .491 − 10 
     CDR − .601   
mu-6 IGHV1-67*1 80.90 10 FR .03 − 12 
     CDR − .110   
mu-7 IGHV1-S22*01 94.62 22 FR − .112 − 15 
     CDR − .009   
mu-8 IGHV14-3*02 98.61 − FR .329 11 
     CDR − 1.278   
mu-9 IGHV1-18*02 88.19 FR 18 10 .107 12 
     CDR .369   
mu-10 IGHV1-S81*02 95.83 10 FR − .23 14 
     CDR − .156   
mu-11 IGHV1-74*01 86.81 10 FR 17 .12 − 12 
     CDR 10 .034   

MALT indicates mucosa-associated lymphoid tissue; FR, framework region; CDR, complementary determining region; R, replacement; S, silent; and mu, murine.

*

VH gene sequences deviating more than 2% from the corresponding germline gene were defined as somatically mutated.

Presence or absence of positive selection by antigen in the CDR is denoted by + and −, respectively. Presence or absence of negative selection by antigen in the FR is denoted by + and −, respectively, based on the cutoff ratio of 2.9 for replacement to silent mutations.

The P value was calculated based on the multinomial distribution model and is the probability that excess (for CDR) or scarcity (for FR) of mutations occurred by chance.