Quantitative whole-cell colocalization analysis of CFH in platelets costained with organelle, surface, and cytoplasmic proteins
| Protein (red*) . | CFH (green*) . | n . | Pearson correlation coefficient, mean ± SD (range) . | Manders overlap coefficient, mean ± SD (range) . |
|---|---|---|---|---|
| VWF | CFH | 15 | 0.359 ± 0.090 (0.261-0.543) | 0.409 ± 0.101 (0.289-0.608) |
| Fibrinogen | CFH | 24 | 0.314 ± 0.077 (0.192-0.453) | 0.381 ± 0.080 (0.262-0.524) |
| CD63 | CFH | 15 | 0.217 ± 0.038 (0.158-0.296) | 0.239 ± 0.038 (0.179-0.321) |
| LAMP-1 | CFH | 20 | 0.308 ± 0.064 (0.209-0.491) | 0.349 ± 0.067 (0.251-0.560) |
| CD61 | CFH | 32 | 0.422 ± 0.087 (0.235-0.601)† | 0.490 ± 0.081 (0.317-0.655) |
| β-Actin | CFH | 20 | 0.858 ± 0.022 (0.814-0.893)† | 0.878 ± 0.019 (0.837-0.910)‡ |
| Protein (red*) . | CFH (green*) . | n . | Pearson correlation coefficient, mean ± SD (range) . | Manders overlap coefficient, mean ± SD (range) . |
|---|---|---|---|---|
| VWF | CFH | 15 | 0.359 ± 0.090 (0.261-0.543) | 0.409 ± 0.101 (0.289-0.608) |
| Fibrinogen | CFH | 24 | 0.314 ± 0.077 (0.192-0.453) | 0.381 ± 0.080 (0.262-0.524) |
| CD63 | CFH | 15 | 0.217 ± 0.038 (0.158-0.296) | 0.239 ± 0.038 (0.179-0.321) |
| LAMP-1 | CFH | 20 | 0.308 ± 0.064 (0.209-0.491) | 0.349 ± 0.067 (0.251-0.560) |
| CD61 | CFH | 32 | 0.422 ± 0.087 (0.235-0.601)† | 0.490 ± 0.081 (0.317-0.655) |
| β-Actin | CFH | 20 | 0.858 ± 0.022 (0.814-0.893)† | 0.878 ± 0.019 (0.837-0.910)‡ |