Cell numbers (×103) from the 2-phase cultures
| . | Amplification step . | Differentiation step . | ||
|---|---|---|---|---|
| D3 . | D5 . | E0 . | E3 . | |
| shControl | 100 | 215 ± 1.5 | 100 | 705 ± 4.9 |
| shHMGB2 | 100 | 270 ± 5.0 | 100 | 517 ± 5.5 |
| . | Amplification step . | Differentiation step . | ||
|---|---|---|---|---|
| D3 . | D5 . | E0 . | E3 . | |
| shControl | 100 | 215 ± 1.5 | 100 | 705 ± 4.9 |
| shHMGB2 | 100 | 270 ± 5.0 | 100 | 517 ± 5.5 |
CD34+ cells were plated in the presence of SCF, IL-3, EPO, and dexamethasone then infected with shControl or shHMGB2 48 (D1) and 72 (D2) hours after. Twenty-four hours later (D3) or the day of the induction of erythroid differentiation (D5 = E0) cells were plated at 100 × 103 cells per well and counted in the presence of trypan blue 2 days after.
D indicates day; and E, day after EPO.