Relative concentrations of alternatively spliced forms of UROS in normal and CEP lymphoblasts*
| Source . | 155-bp normal transcript . | 236-bp (+81 nt) variant . | 401-bp (+165/+81 nt) variant . | 513-bp (+81/+277 nt) variant . |
|---|---|---|---|---|
| Normal controls (n = 3) | 81 (58-111) | 14 (10-18) | 5 (4-8) | 0.3 (0.2-0.4) |
| Human UROS ESTs† | 90 | 8 | 2 | 0 |
| CEP patient 3 (n = 2) | 5 (5,5) | 12 (8,19) | 1 (1,1) | 1 (1,2) |
| CEP patient 6 (n = 2) | 14 (5,33) | 27 (12,60) | 4 (1,22) | 10 (3,33) |
| CEP patient 10 (n = 1) | 5 | 27 | 2 | 5 |
| CEP patients Average | 8 (5-19) | 22 (10-40) | 2 (1-11) | 5 (2-17) |
| Source . | 155-bp normal transcript . | 236-bp (+81 nt) variant . | 401-bp (+165/+81 nt) variant . | 513-bp (+81/+277 nt) variant . |
|---|---|---|---|---|
| Normal controls (n = 3) | 81 (58-111) | 14 (10-18) | 5 (4-8) | 0.3 (0.2-0.4) |
| Human UROS ESTs† | 90 | 8 | 2 | 0 |
| CEP patient 3 (n = 2) | 5 (5,5) | 12 (8,19) | 1 (1,1) | 1 (1,2) |
| CEP patient 6 (n = 2) | 14 (5,33) | 27 (12,60) | 4 (1,22) | 10 (3,33) |
| CEP patient 10 (n = 1) | 5 | 27 | 2 | 5 |
| CEP patients Average | 8 (5-19) | 22 (10-40) | 2 (1-11) | 5 (2-17) |
Values are percentage of all transcripts in normal controls (range).
The relative concentrations of the major UROS transcripts were calculated using the ΔΔCt procedure as described in “Quantitative RT-PCR analyses.” The abundance of the each transcript is expressed as a mean percentage of the total amount of normal and alternatively spliced transcripts in the normal controls.
Percentage of each EST found in the 90 ESTs containing human UROS exons 9 and 10 in the March 2006 Assembly of the Human Genome.