Table 2 Prokaryotic expression of... | American Society of Hematology

Table 2

Prokaryotic expression of wild-type and alternatively spliced UROS transcripts

	Specific activity,* U/mg	Net (-vector control), U/mg	Percentage of wild-type
pKK construct
Wild-type (n = 2)	439.5 ± 3.1	431.6	100
pKK vector (n = 3)	7.9 ± 8.8	0	NA
+81-bp insertion (n = 3)	10.5 ± 7.3	2.6	0.6
Mutation p.Ala69Thr† (n = 3)	17.4 ± 0.8	9.5	2.2
pET SUMO construct
Wild-type (n = 5)	2.52 ± 0.13 × 10⁶	NA‡	100
+81-bp insertion (n = 3)	0.105 ± 0.008 × 10⁶	NA‡	4.2 ± 0.3

	Specific activity,* U/mg	Net (-vector control), U/mg	Percentage of wild-type
pKK construct
Wild-type (n = 2)	439.5 ± 3.1	431.6	100
pKK vector (n = 3)	7.9 ± 8.8	0	NA
+81-bp insertion (n = 3)	10.5 ± 7.3	2.6	0.6
Mutation p.Ala69Thr† (n = 3)	17.4 ± 0.8	9.5	2.2
pET SUMO construct
Wild-type (n = 5)	2.52 ± 0.13 × 10⁶	NA‡	100
+81-bp insertion (n = 3)	0.105 ± 0.008 × 10⁶	NA‡	4.2 ± 0.3

NA indicates not applicable.

Mean specific activity ± SD. Activities are for crude extracts for pKK constructs and for pure enzyme for pET SUMO constructs.

The previously published value for residual pKK-expressed activity for this mutation was 1.4% of wild-type.

Not applicable for purified enzyme.