Primer sequences, amplicon sizes, and annealing temperatures of various PCR products
| Gene . | Sense primer . | Antisense primer . | Amplicon size, bp . | Temperature, °C . |
|---|---|---|---|---|
| Transforming growth factor-α (TGF-α) | CAGCATGTGTCTGCCACTCT | GTGTTGTCCCAAGATGCCTT | 350 | 62 |
| Fetal liver tyrosine kinase-3 ligand (Flt3-L) | CAGACCAACATCTCCCACCT | GGTTCCCAACTCTAGCACCA | 518, 580 | 60.8 |
| Interleukin-6 (IL-6) | GTTCTCTGGGAAATCGTGGA | TGGTCTTGGTCCTTAGCCAC | 397 | 57.9 |
| Stem cell factor (SCF) | TCCGAAGAGGCCAGAAACTA | CACGGGTAGCAAGAACAGGT | 501, 585 | 63.0 |
| Vascular endothelial growth factor-α (VEGF-α) | AGCACAGCAGATGTGAATGC | CCTCTTCCTTCATGTCAGGC | 221, 353, 425 | 62 |
| Stromal derived factor-1α (SDF-1α) | TTTCACTCTCGGTCCACCTC | AGATGCTTGACGTTGGCTCT | 215 | 61.5 |
| Hepatocyte growth factor (HGF) | CTACACTCTTGACCCTGACAC | GCCACGATAACAATCTTGTCC | 370 | 59.4 |
| FVIII-A3 domain | TCAAATCCCACCAGTGTTGA | GGGTCCAATTAATCCCGAGT | 637 | 57 |
| β-Actin | AGCCATGTACGTAGCCATCC | CTCTCAGCTGTGGTGGTGAA | 228 | 58.8 |
| Gene . | Sense primer . | Antisense primer . | Amplicon size, bp . | Temperature, °C . |
|---|---|---|---|---|
| Transforming growth factor-α (TGF-α) | CAGCATGTGTCTGCCACTCT | GTGTTGTCCCAAGATGCCTT | 350 | 62 |
| Fetal liver tyrosine kinase-3 ligand (Flt3-L) | CAGACCAACATCTCCCACCT | GGTTCCCAACTCTAGCACCA | 518, 580 | 60.8 |
| Interleukin-6 (IL-6) | GTTCTCTGGGAAATCGTGGA | TGGTCTTGGTCCTTAGCCAC | 397 | 57.9 |
| Stem cell factor (SCF) | TCCGAAGAGGCCAGAAACTA | CACGGGTAGCAAGAACAGGT | 501, 585 | 63.0 |
| Vascular endothelial growth factor-α (VEGF-α) | AGCACAGCAGATGTGAATGC | CCTCTTCCTTCATGTCAGGC | 221, 353, 425 | 62 |
| Stromal derived factor-1α (SDF-1α) | TTTCACTCTCGGTCCACCTC | AGATGCTTGACGTTGGCTCT | 215 | 61.5 |
| Hepatocyte growth factor (HGF) | CTACACTCTTGACCCTGACAC | GCCACGATAACAATCTTGTCC | 370 | 59.4 |
| FVIII-A3 domain | TCAAATCCCACCAGTGTTGA | GGGTCCAATTAATCCCGAGT | 637 | 57 |
| β-Actin | AGCCATGTACGTAGCCATCC | CTCTCAGCTGTGGTGGTGAA | 228 | 58.8 |