Analysis of the amino acid sequences of selected human Fab
| . | JML-1 . | JML-3 . | JML-7 . | JML-13 . |
|---|---|---|---|---|
| Light chain | ||||
| Human germlines* | Vκ 1-39 | Vκ 1-39 | Vκ 1-39 | Vκ 1-39 |
| Jκ 3 | Jκ 3 | Jκ 3 | Jκ 3 | |
| Deviation from Vκ germline† | 0/95 | 2/95 | 1/95 | 3/95 |
| LCDR3 sequence | QQSYSTPFT | QQSYSTPFT | QQSYSTPFT | QQSYSTPFT |
| Sequence identity‡ to JML-1 | 100% | 98% | 99% | 97% |
| Sequence identity to JML-3 | 98% | 100% | 97% | 95% |
| Sequence identity to JML-7 | 99% | 97% | 100% | 96% |
| Sequence identity to JML-13 | 97% | 95% | 96% | 100% |
| Heavy chain | ||||
| Human germlines* | VH 3-9 | VH 3-30 | VH 3-30 | VH 3-30 |
| DH 3-10 | DH 3-10 | DH 3-10 | DH 3-10 | |
| JH 3 | JH 3 | JH 3 | JH 3 | |
| Deviation from VH germline† | 7/98 | 2/98 | 2/98 | 1/98 |
| HCDR3 sequence | GGQTIDI | GGQTIDI | GGQTIDI | GGQTIDI |
| Sequence identity to JML-1 | 100% | 89% | 90% | 88% |
| Sequence identity to JML-3 | 89% | 100% | 99% | 99% |
| Sequence identity to JML-7 | 90% | 99% | 100% | 98% |
| Sequence identity to JML-13 | 88% | 99% | 98% | 100% |
| . | JML-1 . | JML-3 . | JML-7 . | JML-13 . |
|---|---|---|---|---|
| Light chain | ||||
| Human germlines* | Vκ 1-39 | Vκ 1-39 | Vκ 1-39 | Vκ 1-39 |
| Jκ 3 | Jκ 3 | Jκ 3 | Jκ 3 | |
| Deviation from Vκ germline† | 0/95 | 2/95 | 1/95 | 3/95 |
| LCDR3 sequence | QQSYSTPFT | QQSYSTPFT | QQSYSTPFT | QQSYSTPFT |
| Sequence identity‡ to JML-1 | 100% | 98% | 99% | 97% |
| Sequence identity to JML-3 | 98% | 100% | 97% | 95% |
| Sequence identity to JML-7 | 99% | 97% | 100% | 96% |
| Sequence identity to JML-13 | 97% | 95% | 96% | 100% |
| Heavy chain | ||||
| Human germlines* | VH 3-9 | VH 3-30 | VH 3-30 | VH 3-30 |
| DH 3-10 | DH 3-10 | DH 3-10 | DH 3-10 | |
| JH 3 | JH 3 | JH 3 | JH 3 | |
| Deviation from VH germline† | 7/98 | 2/98 | 2/98 | 1/98 |
| HCDR3 sequence | GGQTIDI | GGQTIDI | GGQTIDI | GGQTIDI |
| Sequence identity to JML-1 | 100% | 89% | 90% | 88% |
| Sequence identity to JML-3 | 89% | 100% | 99% | 99% |
| Sequence identity to JML-7 | 90% | 99% | 100% | 98% |
| Sequence identity to JML-13 | 88% | 99% | 98% | 100% |
Based on DNA alignments using IMGT/V-QUEST (http://imgt.cines.fr)
Based on amino acid sequence alignments using IgBLAST (http://www.ncbi.nlm.nih.gov/igblast).
Based on amino acid sequences of the variable domains.