Human Fab library after alloHSCT
. | Post-alloHSCT PBMCs . | Normal PBMCs . |
---|---|---|
Successful primer combinations Vκ | 10/12 (83%) | 12/12 (100%) |
Successful primer combinations Vλ | 41/60 (68%) | 59/60 (98%) |
Successful primer combinations VH | 81/114 (71%) | 95/114 (83%) |
Total of successful primer combinations | 132/186 (71%) | 166/186 (89%) |
Phagemid | pC3C | Not applicable |
Library size | 2.6 × 108 | Not applicable |
E coli strain | XL1-Blue | Not applicable |
Helper phage | VCSM13 | Not applicable |
. | Post-alloHSCT PBMCs . | Normal PBMCs . |
---|---|---|
Successful primer combinations Vκ | 10/12 (83%) | 12/12 (100%) |
Successful primer combinations Vλ | 41/60 (68%) | 59/60 (98%) |
Successful primer combinations VH | 81/114 (71%) | 95/114 (83%) |
Total of successful primer combinations | 132/186 (71%) | 166/186 (89%) |
Phagemid | pC3C | Not applicable |
Library size | 2.6 × 108 | Not applicable |
E coli strain | XL1-Blue | Not applicable |
Helper phage | VCSM13 | Not applicable |