PCR primer pairs used to examine the splicing of various genes
| Gene . | Accession no. . | Primer . | Position on cDNA . | Length, bp . | Position on gDNA . | Length, bp . |
|---|---|---|---|---|---|---|
| β-actin | CR624852 | β-actin-up 5′-CCGTCTTCCCCTCCATCGTG-3′ | 160-179 | 576 | Exon 2 | 1151 |
| β-actin-dw 5′-GGGCGACGTAGCACAGCTTCT-3′ | 715-735 | Exon 4 | ||||
| RNA Polymerase I D | NM_015972 | RNA-up 5′-TGGAAGAGGATCAGGAGCTGG-3′ | 94-114 | 310 | Exon 1 | 1176 |
| RNA-dw 5′-CATGAGCTCATTCAGGCCTCTC-3′ | 383-404 | Exon 2 | ||||
| MRP1 | NM_004996 | MRP-EX23 5′-GTGCGGCTGGAGTGTGTGG-3′ | 3776-3794 | 198 | Exon 23 | 1092 |
| MRP-EX24 5′-CTCCTTGAGCCTCTCCACGG-3′ | 3954-3973 | Exon 24 | ||||
| TS | NM_001071 | TS-U5 5′-CCTGAATCACATCGAGCCAC-3′ | 879-898 | 142 | Exon 6 | 1550 |
| TS-D4 5′-TGGATGCGGATTGTACCCTT-3′ | 1001-1020 | Exon 7 | ||||
| DHFR | NM_000791 | DHFR-EX1 5′-TCGCTAAACTGCATCGTCGC-3′ | 502-521 | 115 | Exon 1 | 460 |
| DHFR-D1 5′-AGGTTGTGGTCATTCTCTGG-3′ | 597-616 | Exon 2 | ||||
| RFC | U19720.1 | RFC2-up 5′-GGGCGTGTTCACCAGCTC-3′ | 581-598 | 576 | Exon 3 | 993 |
| RFC2-dw 5′-GCCAGAAGGAAGACCAGCC-3′ | 1138-1156 | Exon 4 | ||||
| PDGF | M63193 | TP02-up 5′-GCTTCGTGGCCGCTGTGGTG-3′ | 290-309 | 350 | Exon 2 | 1030 |
| TP02-dw 5′-CTGCTCTGGGCTCTGGATGA-3′ | 620-639 | Exon 4 | ||||
| GAPDH | NM_002046 | GAP-up 5′-AGGGGGGAGCCAAAAGGG-3′ | 440-457 | 541 | Exon 6 | 826 |
| GAP-up 5′-GAGGAGTGGGTGTCGCTGTTG-3′ | 960-980 | Exon 8 | ||||
| TNFα | NM_000594 | TNFaG-up 5′-GATCATCTTCTCGAACCCCG-3′ | 402-420 | 301 | Exon 3 | 602 |
| TNFaG-dw 5′-TGGCAGGGGCTCTTGATG-3′ | 685-702 | Exon 4 | ||||
| UBTF | NM_014233 | UBTF-up 5′-TGGTCCCAGGAAGACATGCTG-3′ | 131-151 | 356 | Exon 2 | 991 |
| UBTF-dw 5′-AGGTTGCTCATCTCAGGGTGG-3′ | 466-486 | Exon 4 | ||||
| Plexin A1 | NM_032242 | Plex-up 5′-AGCTCCCCACTCATCCTCAAG-3′ | 3431-3451 | 362 | Exon 17 | 847 |
| Plex-dw 5′-TCCATCTGGAGCTGCAGCC-3′ | 3774-3792 | Exon 19 |
| Gene . | Accession no. . | Primer . | Position on cDNA . | Length, bp . | Position on gDNA . | Length, bp . |
|---|---|---|---|---|---|---|
| β-actin | CR624852 | β-actin-up 5′-CCGTCTTCCCCTCCATCGTG-3′ | 160-179 | 576 | Exon 2 | 1151 |
| β-actin-dw 5′-GGGCGACGTAGCACAGCTTCT-3′ | 715-735 | Exon 4 | ||||
| RNA Polymerase I D | NM_015972 | RNA-up 5′-TGGAAGAGGATCAGGAGCTGG-3′ | 94-114 | 310 | Exon 1 | 1176 |
| RNA-dw 5′-CATGAGCTCATTCAGGCCTCTC-3′ | 383-404 | Exon 2 | ||||
| MRP1 | NM_004996 | MRP-EX23 5′-GTGCGGCTGGAGTGTGTGG-3′ | 3776-3794 | 198 | Exon 23 | 1092 |
| MRP-EX24 5′-CTCCTTGAGCCTCTCCACGG-3′ | 3954-3973 | Exon 24 | ||||
| TS | NM_001071 | TS-U5 5′-CCTGAATCACATCGAGCCAC-3′ | 879-898 | 142 | Exon 6 | 1550 |
| TS-D4 5′-TGGATGCGGATTGTACCCTT-3′ | 1001-1020 | Exon 7 | ||||
| DHFR | NM_000791 | DHFR-EX1 5′-TCGCTAAACTGCATCGTCGC-3′ | 502-521 | 115 | Exon 1 | 460 |
| DHFR-D1 5′-AGGTTGTGGTCATTCTCTGG-3′ | 597-616 | Exon 2 | ||||
| RFC | U19720.1 | RFC2-up 5′-GGGCGTGTTCACCAGCTC-3′ | 581-598 | 576 | Exon 3 | 993 |
| RFC2-dw 5′-GCCAGAAGGAAGACCAGCC-3′ | 1138-1156 | Exon 4 | ||||
| PDGF | M63193 | TP02-up 5′-GCTTCGTGGCCGCTGTGGTG-3′ | 290-309 | 350 | Exon 2 | 1030 |
| TP02-dw 5′-CTGCTCTGGGCTCTGGATGA-3′ | 620-639 | Exon 4 | ||||
| GAPDH | NM_002046 | GAP-up 5′-AGGGGGGAGCCAAAAGGG-3′ | 440-457 | 541 | Exon 6 | 826 |
| GAP-up 5′-GAGGAGTGGGTGTCGCTGTTG-3′ | 960-980 | Exon 8 | ||||
| TNFα | NM_000594 | TNFaG-up 5′-GATCATCTTCTCGAACCCCG-3′ | 402-420 | 301 | Exon 3 | 602 |
| TNFaG-dw 5′-TGGCAGGGGCTCTTGATG-3′ | 685-702 | Exon 4 | ||||
| UBTF | NM_014233 | UBTF-up 5′-TGGTCCCAGGAAGACATGCTG-3′ | 131-151 | 356 | Exon 2 | 991 |
| UBTF-dw 5′-AGGTTGCTCATCTCAGGGTGG-3′ | 466-486 | Exon 4 | ||||
| Plexin A1 | NM_032242 | Plex-up 5′-AGCTCCCCACTCATCCTCAAG-3′ | 3431-3451 | 362 | Exon 17 | 847 |
| Plex-dw 5′-TCCATCTGGAGCTGCAGCC-3′ | 3774-3792 | Exon 19 |