Table 2 Summary of Notch1 single point... | American Society of Hematology

Table 2

Summary of Notch1 single point mutations found in T-ALL

Mutation	Reference	Location	% buried	Ligand-independent reporter activity	Urea dissociation propensity	Putative classification
L1575P	18	β1	99		+++	Core
L1575Q	19
V1577E	19	β1	100	++	+++	Core
V1579G	19	β1	100			Core
L1586P	18	α1	100			Core
L1586R	19
R1587P	19	α1	50			Partially exposed
F1593S	18	α1	100	+++	+++	Core
F1593C	19
L1594P	18	α1	93	++	++	Core
L1597H	18,27	α1	100	+++	+++	Core
R1599P	18	α1	45	+++	+++	Partially exposed
L1601P	18	α1	99		++++	Core
L1601Q	19
Q1615K	19	Loop β2, β3	74			Partially exposed
I1617T	18,27	β3	95	+	++	Core
I1617N	18				+++
Y1620N	19	Furin loop	66			Partially exposed
V1677D	18	β4	100	+	++++	Core
L1679P	18	β4	100	+	++++	Core
L1679Q	24
I1681N	18	β4	98	++	+++	Core
C1686F	19	Cysteine-knuckle	55			Partially exposed
A1697D	22	α3	97			Core
A1702P	18	α3	94	+	+	Interface
L1710P	20	α3	92			Interface
I1719T	18,27	Loop α3, β5	86	+	++	Interface
H1545P	21	LNR-C	17	++	+	Partially exposed

Mutation	Reference	Location	% buried	Ligand-independent reporter activity	Urea dissociation propensity	Putative classification
L1575P	18	β1	99		+++	Core
L1575Q	19
V1577E	19	β1	100	++	+++	Core
V1579G	19	β1	100			Core
L1586P	18	α1	100			Core
L1586R	19
R1587P	19	α1	50			Partially exposed
F1593S	18	α1	100	+++	+++	Core
F1593C	19
L1594P	18	α1	93	++	++	Core
L1597H	18,27	α1	100	+++	+++	Core
R1599P	18	α1	45	+++	+++	Partially exposed
L1601P	18	α1	99		++++	Core
L1601Q	19
Q1615K	19	Loop β2, β3	74			Partially exposed
I1617T	18,27	β3	95	+	++	Core
I1617N	18				+++
Y1620N	19	Furin loop	66			Partially exposed
V1677D	18	β4	100	+	++++	Core
L1679P	18	β4	100	+	++++	Core
L1679Q	24
I1681N	18	β4	98	++	+++	Core
C1686F	19	Cysteine-knuckle	55			Partially exposed
A1697D	22	α3	97			Core
A1702P	18	α3	94	+	+	Interface
L1710P	20	α3	92			Interface
I1719T	18,27	Loop α3, β5	86	+	++	Interface
H1545P	21	LNR-C	17	++	+	Partially exposed

+ represents relative strength of measured effect as reported in listed references.