Puc18 sequence
| . | Puc18 sequence . |
|---|---|
| 1 | tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca |
| 61 | cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg |
| 121 | ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc |
| 181 | accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc |
| 241 | attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat |
| 301 | tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt |
| 361 | tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcaggtcg |
| 421 | actctagagg atccccgggt accgagctcg aattcgtaat catggtcata gctgtttcct |
| 481 | gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt |
| 541 | aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc |
| 601 | gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg |
| 661 | agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg |
| 721 | gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca |
| . | Puc18 sequence . |
|---|---|
| 1 | tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca |
| 61 | cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg |
| 121 | ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc |
| 181 | accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc |
| 241 | attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat |
| 301 | tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt |
| 361 | tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcaggtcg |
| 421 | actctagagg atccccgggt accgagctcg aattcgtaat catggtcata gctgtttcct |
| 481 | gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt |
| 541 | aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc |
| 601 | gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg |
| 661 | agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg |
| 721 | gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca |
Forward primer: cggcatcaga gcagattgta; reverse primer: gg cggtaatacg gttatcca; EcoR1 Site: g aattcg.