“Stereotyped” amino acid changes
| Sequence (subset no.) . | Change* . | CLL-subset . | CLL-heterogeneous . | Non-CLL† . |
|---|---|---|---|---|
| IGLV3-21 (no. 2) | ||||
| IMGT-LCDR3, codon 110 | S→G | 17/37 | 14/55 | 9/197 |
| IGKV2-30 (no. 4) | ||||
| IMGT-KCDR1, codon 31 | Y→H | 10/15 | 1/17 | 6/62 |
| IMGT-KFR2, codon 43 | Q→H | 4/15 | 2/17 | 2/62 |
| IMGT-KFR3, codon 66 | N→D | 8/15 | 3/17 | 1/62 |
| Sequence (subset no.) . | Change* . | CLL-subset . | CLL-heterogeneous . | Non-CLL† . |
|---|---|---|---|---|
| IGLV3-21 (no. 2) | ||||
| IMGT-LCDR3, codon 110 | S→G | 17/37 | 14/55 | 9/197 |
| IGKV2-30 (no. 4) | ||||
| IMGT-KCDR1, codon 31 | Y→H | 10/15 | 1/17 | 6/62 |
| IMGT-KFR2, codon 43 | Q→H | 4/15 | 2/17 | 2/62 |
| IMGT-KFR3, codon 66 | N→D | 8/15 | 3/17 | 1/62 |
The frequency of changes among mutated sequences using the same IGKV or IGLV gene was recorded in CLL cases with stereotyped BCRs (cases belonging to subsets, numbered as in References 11,12), CLL cases with heterogeneous BCRs, and IGKV/IGLV sequences from normal or autoreactive clones (non-CLL).
BCR indicates B-cell receptor; and CLL, chronic lymphocytic leukemia.
All amino acid changes listed in Table 2 were significantly more frequent among cases with stereotyped BCRs and, therefore, could be considered “subset-biased.”
A complete list of the non-CLL (external) sequences carrying the indicated changes is given in Table S27.