Primer sets of human S1P receptors and β-actin
| Gene . | Sequence . | Product size, bp . |
|---|---|---|
| S1P1 | ||
| Forward | 5′-TGCGGGAAGGGAGTATGTTT-3′ | |
| Reverse | 5′-CGATGGCGAGGAGACTGAAC-3′ | 60 |
| S1P2 | ||
| Forward | 5′-GCCTCTCTACGCCAAGCATTA-3′ | |
| Reverse | 5′-TTGAGCGGACCACGCAGTA-3′ | 107 |
| S1P3 | ||
| Forward | 5′-TGATTGTGGTGAGCGTGTTCA-3′ | |
| Reverse | 5′-GGCCACATCAATGAGGAAGAG-3′ | 68 |
| β-actin | ||
| Forward | 5′-TCTACAATGAGCTGCGTGTG-3′ | |
| Reverse | 5′-ATGGCTGGGGTGTTGAAG-3′ | 127 |
| Gene . | Sequence . | Product size, bp . |
|---|---|---|
| S1P1 | ||
| Forward | 5′-TGCGGGAAGGGAGTATGTTT-3′ | |
| Reverse | 5′-CGATGGCGAGGAGACTGAAC-3′ | 60 |
| S1P2 | ||
| Forward | 5′-GCCTCTCTACGCCAAGCATTA-3′ | |
| Reverse | 5′-TTGAGCGGACCACGCAGTA-3′ | 107 |
| S1P3 | ||
| Forward | 5′-TGATTGTGGTGAGCGTGTTCA-3′ | |
| Reverse | 5′-GGCCACATCAATGAGGAAGAG-3′ | 68 |
| β-actin | ||
| Forward | 5′-TCTACAATGAGCTGCGTGTG-3′ | |
| Reverse | 5′-ATGGCTGGGGTGTTGAAG-3′ | 127 |