Spontaneous mutagenesis rate
| . | Cell line . | ||
|---|---|---|---|
| Ba/F3-EPOR . | Ba/F3-JAK2wt . | Ba/F3-JAK2V617F . | |
| HPRT locus | |||
| Resistance | 6-TG | 6-TG | 6-TG |
| Mutation frequency | 8.5 × 10−5 ± 0.2 × 10−5 | 1.7 × 10−5 ± 4.3 × 10−5 | 7.4 × 10−3 ± 5.9 × 10−3 |
| Mutation rate (rate/cell per generation)40 | 3.1 × 10−5 ± 5.0 × 10−5 | 1.6 × 10−5 ± 1.9 × 10−5 | 1.1 × 10−3 ± 0.9 × 10−3 |
| Mutation rate (locus/cell per generation)39 | 2.0 × 10−5 ± 0.6 × 10−5 | 6.0 × 10−5 ± 0.8 × 10−5 | NA |
| Na/K-ATPase locus | |||
| Resistance | Ouabain | Ouabain | Ouabain |
| Mutation frequency | 2.2 × 10−5 ± 5.3 × 10−5 | 1.5 × 10−5 ± 3.7 × 10−5 | 3.9 × 10−4 ± 5.5 × 10−4 |
| Mutation rate (rate/cell per generation)40 | 1.7 × 10−5 ± 2.1 × 10−5 | 1.6 × 10−5 ± 1.8 × 10−5 | 1.2 × 10−4 ± 1.5 × 10−4 |
| Mutation rate (locus/cell per generation)39 | 7.1 × 10−6 ± 0.8 × 10−6 | 5.3 × 10−6 ± 0.9 × 10−6 | 7.2 × 10−5 ± 0.5 × 10−5 |
| . | Cell line . | ||
|---|---|---|---|
| Ba/F3-EPOR . | Ba/F3-JAK2wt . | Ba/F3-JAK2V617F . | |
| HPRT locus | |||
| Resistance | 6-TG | 6-TG | 6-TG |
| Mutation frequency | 8.5 × 10−5 ± 0.2 × 10−5 | 1.7 × 10−5 ± 4.3 × 10−5 | 7.4 × 10−3 ± 5.9 × 10−3 |
| Mutation rate (rate/cell per generation)40 | 3.1 × 10−5 ± 5.0 × 10−5 | 1.6 × 10−5 ± 1.9 × 10−5 | 1.1 × 10−3 ± 0.9 × 10−3 |
| Mutation rate (locus/cell per generation)39 | 2.0 × 10−5 ± 0.6 × 10−5 | 6.0 × 10−5 ± 0.8 × 10−5 | NA |
| Na/K-ATPase locus | |||
| Resistance | Ouabain | Ouabain | Ouabain |
| Mutation frequency | 2.2 × 10−5 ± 5.3 × 10−5 | 1.5 × 10−5 ± 3.7 × 10−5 | 3.9 × 10−4 ± 5.5 × 10−4 |
| Mutation rate (rate/cell per generation)40 | 1.7 × 10−5 ± 2.1 × 10−5 | 1.6 × 10−5 ± 1.8 × 10−5 | 1.2 × 10−4 ± 1.5 × 10−4 |
| Mutation rate (locus/cell per generation)39 | 7.1 × 10−6 ± 0.8 × 10−6 | 5.3 × 10−6 ± 0.9 × 10−6 | 7.2 × 10−5 ± 0.5 × 10−5 |
Six cultures were used in 5 of the treatments, and 5 for HPRT with 6-TG resistance.
NA indicates not applicable.