Primers used for PCR
| Genes and primers . | Sequence . |
|---|---|
| VCAM-1 | |
| 5′ | GAACACTTTTCCCAGACACTTTTCACGTGG |
| 3′ | TAAGGATCTGGGTTTTATAAATAATGTCTC |
| VECAD | |
| 5′ | GAACGAGGACAGCAACTTCACCCTCATA |
| 3′ | CTGACACATCATAGCTGGTGGTGTCCAT |
| Fibronectin | |
| 5′ | GGACCCCGCTAAACTCTTCCACCATTAT |
| 3′ | GGAGAGGGATGCTCTCATGTTGTTCGTA |
| UPAR | |
| 5′ | GCTTCGGGAATGGCAAGATGATAGAGAG |
| 3′ | CAGTGGGTGTAGTTGCAACACTTCAGGA |
| PECAM-1 | |
| 5′ | CACAGATAAGCCCACCAGAGACATGGA |
| 3′ | TCTCGCTGTTGGAGTTCAGAAGTGGAG |
| Flk-1 | |
| 5′ | AGGACCAAGGCGACTATGTTTGCTCTG |
| 3′ | ACGGTCCGTACGAGAATGACAAGAAGG |
| Tie-2 | |
| 5′ | CGTCAACAAGCATCCTTCCTACCTGCTA |
| 3′ | TGCATCCTTCTGGTCCACTACACCTTTC |
| HIF-1α | |
| 5′ | CAAGATCTCGGCGAACGAAAGAGTCTGA |
| 3′ | GAAGCACCTTCCACGTTGCTGACTTGAT |
| HIF-2α | |
| 5′ | CAGCTCAGAGCTGAGGAAGG |
| 3′ | GTTGTAGACTCACTTGCC |
| Epo | |
| 5′ | TGGCACCCTGCTGCTTTTACTCTCCTT |
| 3′ | CTGGAGGCGACATCAATTCCTTCTGAG |
| β-actin | |
| 5′ | GTCGTACCACAGGCATTGTGATGGACT |
| 3′ | CACCAGACAGCACTGTGTTGGCATAGA |
| Genes and primers . | Sequence . |
|---|---|
| VCAM-1 | |
| 5′ | GAACACTTTTCCCAGACACTTTTCACGTGG |
| 3′ | TAAGGATCTGGGTTTTATAAATAATGTCTC |
| VECAD | |
| 5′ | GAACGAGGACAGCAACTTCACCCTCATA |
| 3′ | CTGACACATCATAGCTGGTGGTGTCCAT |
| Fibronectin | |
| 5′ | GGACCCCGCTAAACTCTTCCACCATTAT |
| 3′ | GGAGAGGGATGCTCTCATGTTGTTCGTA |
| UPAR | |
| 5′ | GCTTCGGGAATGGCAAGATGATAGAGAG |
| 3′ | CAGTGGGTGTAGTTGCAACACTTCAGGA |
| PECAM-1 | |
| 5′ | CACAGATAAGCCCACCAGAGACATGGA |
| 3′ | TCTCGCTGTTGGAGTTCAGAAGTGGAG |
| Flk-1 | |
| 5′ | AGGACCAAGGCGACTATGTTTGCTCTG |
| 3′ | ACGGTCCGTACGAGAATGACAAGAAGG |
| Tie-2 | |
| 5′ | CGTCAACAAGCATCCTTCCTACCTGCTA |
| 3′ | TGCATCCTTCTGGTCCACTACACCTTTC |
| HIF-1α | |
| 5′ | CAAGATCTCGGCGAACGAAAGAGTCTGA |
| 3′ | GAAGCACCTTCCACGTTGCTGACTTGAT |
| HIF-2α | |
| 5′ | CAGCTCAGAGCTGAGGAAGG |
| 3′ | GTTGTAGACTCACTTGCC |
| Epo | |
| 5′ | TGGCACCCTGCTGCTTTTACTCTCCTT |
| 3′ | CTGGAGGCGACATCAATTCCTTCTGAG |
| β-actin | |
| 5′ | GTCGTACCACAGGCATTGTGATGGACT |
| 3′ | CACCAGACAGCACTGTGTTGGCATAGA |