CD200 expression at the surface of myeloma cells
. | CD200 labeling . | . | |
|---|---|---|---|
| Patient no. . | % . | MFI . | |
| 1 | 84.8 | 2273 | |
| 2 | 90.4 | 4347 | |
| 3 | 88.8 | 986 | |
| 4 | 97.7 | 2023 | |
| 5 | 80.0 | 1219 | |
| 6 | 98.1 | 1155 | |
| 7 | < 5.0 | — | |
| 8 | 99.6 | 8038 | |
| 9 | < 5.0 | — | |
| 10 | 95.9 | 804 | |
| 11 | 95.6 | 832 | |
| 12 | 30.7 | 364 | |
| 13 | 99.9 | 1259 | |
| 14 | 62.0 | 601 | |
| 15 | 97.8 | 442 | |
. | CD200 labeling . | . | |
|---|---|---|---|
| Patient no. . | % . | MFI . | |
| 1 | 84.8 | 2273 | |
| 2 | 90.4 | 4347 | |
| 3 | 88.8 | 986 | |
| 4 | 97.7 | 2023 | |
| 5 | 80.0 | 1219 | |
| 6 | 98.1 | 1155 | |
| 7 | < 5.0 | — | |
| 8 | 99.6 | 8038 | |
| 9 | < 5.0 | — | |
| 10 | 95.9 | 804 | |
| 11 | 95.6 | 832 | |
| 12 | 30.7 | 364 | |
| 13 | 99.9 | 1259 | |
| 14 | 62.0 | 601 | |
| 15 | 97.8 | 442 | |
CD200 expression by primary MMCs was determined using a double labeling of primary MMCs with an FITC-conjugated anti-CD138 MoAb and a PE-conjugated anti-CD200 MoAb, or with FITC- or PE-conjugated isotype-matched control antibodies. The fluorescence intensity was determined with a FACScan device, setting up the mean fluorescence intensity obtained with the control antibodies between 4 and 6.
— indicates no labeling of anti-CD20 antibody.