Table 1. Frequency of TCR Vα-chain... | American Society of Hematology

Table 1.

Frequency of TCR Vα-chain expression on CD4⁺CD25⁺ and CD25^- lymphocytes

	Thymus							PBMCs
	CD25^-	CD25⁺	P	CI	CD25^-	CD25⁺	P	CI
Vα2⁺, %	1.1 ± 0.7	2.8 ± 0.2	.002	1.0-2.3	3.1 ± 0.6	5.0 ± 0.6	< .001	1.2-2.6
Vα12⁺, %	2.8 ± 0.3	3.9 ± 1.0	.032	0.2-2.0	2.4 ± 0.6	3.1 ± 0.9	< .001	0.4-0.9
Vα24⁺, %	ND	ND	ND	ND	0.3 ± 0.1	0.5 ± 0.1	.002	0.1-0.3
Vα2⁺12⁺, %	0.03 ± 0.02	0.09 ± 0.01	.012	0.03-0.09	0.02 ± 0.01	0.10 ± 0.07	.011	0.02-0.12
Vα12⁺ of Vα2⁺ cells, %	1.5 ± 0.6	4.1 ± 1.2	.009	1.3-4.1	0.4 ± 0.2	1.5 ± 0.7	.002	0.6-1.6
Control mAb, single positive, %*	ND	ND	ND	ND	0.1 ± 0.1	0.1 ± 0.2	NS	0-0.1
Control mAb, dual positive, %†	ND	ND	ND	ND	0.01 ± 0.01	0.01 ± 0.01	NS	-0.01-0.01
Vα12⁺CD3^-, %	ND	ND	ND	ND	0.1 ± 0.2	0.1 ± 0.1	NS	-0.1-0

	Thymus							PBMCs
	CD25^-	CD25⁺	P	CI	CD25^-	CD25⁺	P	CI
Vα2⁺, %	1.1 ± 0.7	2.8 ± 0.2	.002	1.0-2.3	3.1 ± 0.6	5.0 ± 0.6	< .001	1.2-2.6
Vα12⁺, %	2.8 ± 0.3	3.9 ± 1.0	.032	0.2-2.0	2.4 ± 0.6	3.1 ± 0.9	< .001	0.4-0.9
Vα24⁺, %	ND	ND	ND	ND	0.3 ± 0.1	0.5 ± 0.1	.002	0.1-0.3
Vα2⁺12⁺, %	0.03 ± 0.02	0.09 ± 0.01	.012	0.03-0.09	0.02 ± 0.01	0.10 ± 0.07	.011	0.02-0.12
Vα12⁺ of Vα2⁺ cells, %	1.5 ± 0.6	4.1 ± 1.2	.009	1.3-4.1	0.4 ± 0.2	1.5 ± 0.7	.002	0.6-1.6
Control mAb, single positive, %*	ND	ND	ND	ND	0.1 ± 0.1	0.1 ± 0.2	NS	0-0.1
Control mAb, dual positive, %†	ND	ND	ND	ND	0.01 ± 0.01	0.01 ± 0.01	NS	-0.01-0.01
Vα12⁺CD3^-, %	ND	ND	ND	ND	0.1 ± 0.2	0.1 ± 0.1	NS	-0.1-0

Nonspecific background staining was tested by using irrelevant isotype-matched FITC-or PE-labeled mouse IgG. The frequency of cells positive for (*) either FITC or PE or (†) both are shown. The average number of fluorescence-activated cell sorting (FACS) events in the lymphocyte gate was as follows: VαX⁺ cells, 120 400; Vα2⁺12⁺ cells, 698 100; Vα12⁺ of Vα2⁺ cells, 149 400.

CI indicates 95% confidence interval for the difference between CD25^- and CD25⁺ cells; ND, not determined; and NS, not significant.

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