Distribution of transcript and protein abundance
Relative abundance, decile . | Transcripts, % . | Proteins, % . | Average no. of peptide hits per protein . |
|---|---|---|---|
| 1 | 20.6 | 85.1 | 5.8 |
| 2 | 28.4 | 7.8 | 39.2 |
| 3 | 22.0 | 2.8 | 66.1 |
| 4 | 9.9 | 2.1 | 95.8 |
| 5 | 10.6 | 1.4 | 111.0 |
| 6 | 2.8 | 0 | — |
| 7 | 1.4 | 0 | — |
| 8 | 2.1 | 0 | — |
| 9 | 1.4 | 0 | — |
| 10 | 0.7 | 0.7 | 257 |
Relative abundance, decile . | Transcripts, % . | Proteins, % . | Average no. of peptide hits per protein . |
|---|---|---|---|
| 1 | 20.6 | 85.1 | 5.8 |
| 2 | 28.4 | 7.8 | 39.2 |
| 3 | 22.0 | 2.8 | 66.1 |
| 4 | 9.9 | 2.1 | 95.8 |
| 5 | 10.6 | 1.4 | 111.0 |
| 6 | 2.8 | 0 | — |
| 7 | 1.4 | 0 | — |
| 8 | 2.1 | 0 | — |
| 9 | 1.4 | 0 | — |
| 10 | 0.7 | 0.7 | 257 |
In this prototype experiment, solubilized platelet proteins were analyzed by μLC-MS/MS, and protein abundance was quantified from the number of peptide hits per individual protein (in this experiment, peptide hits per protein ranged from 1 to 257). Relative protein abundance was determined by the number of peptide hits per protein, and rank-ordered proteins were assigned to deciles based on abundance (1, least abundant; 10, most abundant). The percentage of proteins per decile and the average number of peptide hits per protein are also delineated. Note that most proteins (>85%) were skewed toward those of low abundance, represented by a small number of peptide hits, though transcript expression is distributed more evenly (abundance of corresponding transcripts was determined as previously reported90 ).
—indicates not applicable.