Analysis of Stable A20-2J Transfectants
| Construct . | Neo R . | CAT Expression . | ||
|---|---|---|---|---|
| Basal . | IFN-α/β . | IFN-γ . | ||
| pCAT-5 (−900) | 11 | 9 | 9 | 0 |
| pCAT-4 (−420) | 5 | 4 | 4 | 0 |
| pCAT-2 (−167) | 4 | 4 | 4 | 0 |
| pCAT-3 (−138) | 4 | 4 | 4 | 0 |
| pCAT-8 (−113) | 5 | 4 | 4 | 0 |
| pCAT-1 (−81) | 3 | 3 | 0 | 0 |
| TK-CAT | 9 | 7 | 0 | 0 |
| Tri-TK-CAT | 6 | 6 | 6 | 0 |
| Ly-6E expression | ||||
| HMGI-C antisense | 11 | 0 | 0 | 5 |
| Construct . | Neo R . | CAT Expression . | ||
|---|---|---|---|---|
| Basal . | IFN-α/β . | IFN-γ . | ||
| pCAT-5 (−900) | 11 | 9 | 9 | 0 |
| pCAT-4 (−420) | 5 | 4 | 4 | 0 |
| pCAT-2 (−167) | 4 | 4 | 4 | 0 |
| pCAT-3 (−138) | 4 | 4 | 4 | 0 |
| pCAT-8 (−113) | 5 | 4 | 4 | 0 |
| pCAT-1 (−81) | 3 | 3 | 0 | 0 |
| TK-CAT | 9 | 7 | 0 | 0 |
| Tri-TK-CAT | 6 | 6 | 6 | 0 |
| Ly-6E expression | ||||
| HMGI-C antisense | 11 | 0 | 0 | 5 |
Quantitative stimulation of CAT activity was performed by densitometric scanning of autoradiographs and only those clones which showed at least threefold induction were considered inducible. Estimation of Ly-6E expression was performed by FACS analysis.
Abbreviations: NeoR, total number of clones analyzed; basal, the number of clones that expressed basal CAT activity; IFN-γ, the number of clones showing IFN-γ–inducible CAT activity; IFN-α/β, the number of clones showing IFN-α/β–inducible CAT activity.