Analysis of Stable A20-2J Transfectants
| Construct . | Neo R . | Basal . | IFN-α/β . | IFN-γ . |
|---|---|---|---|---|
| Ly-6A/E | 8 | 0 | 8 | 8 |
| Ly-6A/E.3 | 5 | 0 | 4 | 4 |
| Ly-6A/E.2 | 11 | 0 | 0 | 0 |
| Ly-6A/E.19 | 13 | 0 | 7 | 7 |
| Ly-6A/E.20 | 11 | 0 | 0 | 0 |
| Ly-6A/E.18 | 8 | 0 | 0 | 0 |
| Construct . | Neo R . | Basal . | IFN-α/β . | IFN-γ . |
|---|---|---|---|---|
| Ly-6A/E | 8 | 0 | 8 | 8 |
| Ly-6A/E.3 | 5 | 0 | 4 | 4 |
| Ly-6A/E.2 | 11 | 0 | 0 | 0 |
| Ly-6A/E.19 | 13 | 0 | 7 | 7 |
| Ly-6A/E.20 | 11 | 0 | 0 | 0 |
| Ly-6A/E.18 | 8 | 0 | 0 | 0 |
Estimation of Ly-6A expression was performed by FACS analysis and those clones that showed at least threefold induction were considered inducible.
Abbreviations: NeoR, total number of clones analyzed; basal, the number of clones that expressed basal levels of Ly-6A antigen; IFN-γ, the number of clones showing IFN-γ–inducible Ly-6A; IFN-α/β, the number of clones showing IFN-α/β–inducible Ly-6A expression.