Effects of Vitamin D3 Analogs on Clonal Growth of HL-60 Cells
| Chemical Name . | Code Name . | ED50 (mol/L) . |
|---|---|---|
| 1,25-(OH)2-D3 | 1,25D3 | 1 × 10−9 |
| 1,25S-(OH)2-16,23-diene-26-F3-19nor D3 | Ro 25-9716 | 4 × 10−11 |
| 1,25-(OH)2-16-ene-23-yne-26-F3-19nor D3 | Ro 26-3016 | 6 × 10−11 |
| 1,25-(OH)2-16-ene-19-nor D3 | Ro 25-7290 | 3 × 10−10 |
| 1,25-(OH)2-16,23Z-diene-19-nor D3 | Ro 25-7463 | 4 × 10−10 |
| 1,25-(OH)2-22E,24E-diene-24,26,27-trishomo-19-nor D3 | Ro 25-8584 | 5 × 10−10 |
| 1,25-(OH)2-16-ene-23-yne-19-nor D3 | Ro 25-7856 | 5 × 10−10 |
| 1,25-(OH)2-16,23Z-diene-26,27-F6-19-nor-20-epi D3 | Ro 26-3884 | 5 × 10−10 |
| 1,25-(OH)2-16,23E-diene-19-nor D3 | Ro 25-7555 | 6 × 10−10 |
| 1,25-(OH)2-16,23E-diene-26,27-F6D3 | Ro 25-5818 | 6 × 10−10 |
| Chemical Name . | Code Name . | ED50 (mol/L) . |
|---|---|---|
| 1,25-(OH)2-D3 | 1,25D3 | 1 × 10−9 |
| 1,25S-(OH)2-16,23-diene-26-F3-19nor D3 | Ro 25-9716 | 4 × 10−11 |
| 1,25-(OH)2-16-ene-23-yne-26-F3-19nor D3 | Ro 26-3016 | 6 × 10−11 |
| 1,25-(OH)2-16-ene-19-nor D3 | Ro 25-7290 | 3 × 10−10 |
| 1,25-(OH)2-16,23Z-diene-19-nor D3 | Ro 25-7463 | 4 × 10−10 |
| 1,25-(OH)2-22E,24E-diene-24,26,27-trishomo-19-nor D3 | Ro 25-8584 | 5 × 10−10 |
| 1,25-(OH)2-16-ene-23-yne-19-nor D3 | Ro 25-7856 | 5 × 10−10 |
| 1,25-(OH)2-16,23Z-diene-26,27-F6-19-nor-20-epi D3 | Ro 26-3884 | 5 × 10−10 |
| 1,25-(OH)2-16,23E-diene-19-nor D3 | Ro 25-7555 | 6 × 10−10 |
| 1,25-(OH)2-16,23E-diene-26,27-F6D3 | Ro 25-5818 | 6 × 10−10 |
Each compound was tested at log intervals in dose-response studies (10−11 to 10−7 mol/L); means ± SD were determined and plotted on semilog paper. The dose that produced 50% inhibition of colony formation (ED50) was calculated. Each experimental point had three plates per experiment; the SD was always less than 10%.