Percent Vβ6+ and Vβ3+Cells in BAL Fluid and Splenic Lymphocyte Populations
| Group . | % αβ TCR+Cells . | |||
|---|---|---|---|---|
| BAL . | Spleen . | |||
| Vβ6+ . | Vβ3+ . | Vβ6+ . | Vβ3+ . | |
| Naive animals | ||||
| CBA | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.5 ± 0.0 | 0.3 ± 0.1 |
| B10.BR | 10.2 ± 0.1 | 5.3 ± 0.1 | 10.3 ± 0.6 | 5.5 ± 0.4 |
| 6 weeks after BMT | ||||
| Syngeneic | 0.5 ± 0.2 | 0.2 ± 0.1 | 0.7 ± 0.1 | 0.5 ± 0.1 |
| Allo (TCD) | 21.4 ± 3.2*,† | 4.7 ± 0.6* | 5.5 ± 0.8*,† | 2.1 ± 0.1*,† |
| Allo (+T cells) | 37.1 ± 3.9‡ | 6.3 ± 0.6‡ | 10.3 ± 0.6‡ | 3.6 ± 0.3‡ |
| Group . | % αβ TCR+Cells . | |||
|---|---|---|---|---|
| BAL . | Spleen . | |||
| Vβ6+ . | Vβ3+ . | Vβ6+ . | Vβ3+ . | |
| Naive animals | ||||
| CBA | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.5 ± 0.0 | 0.3 ± 0.1 |
| B10.BR | 10.2 ± 0.1 | 5.3 ± 0.1 | 10.3 ± 0.6 | 5.5 ± 0.4 |
| 6 weeks after BMT | ||||
| Syngeneic | 0.5 ± 0.2 | 0.2 ± 0.1 | 0.7 ± 0.1 | 0.5 ± 0.1 |
| Allo (TCD) | 21.4 ± 3.2*,† | 4.7 ± 0.6* | 5.5 ± 0.8*,† | 2.1 ± 0.1*,† |
| Allo (+T cells) | 37.1 ± 3.9‡ | 6.3 ± 0.6‡ | 10.3 ± 0.6‡ | 3.6 ± 0.3‡ |
CBA mice received syngeneic or allogeneic BMT with and without the addition of donor T cells as in Fig 1. Vβ6+ and Vβ3+ T cell populations were analyzed in BAL fluid and spleens from naive mice and mice 6 weeks after BMT as described in Materials and Methods. Data are expressed as mean percent ± SEM (n = 8 to 12 per BMT group; n = 3 per naive group).
P < .01 Allo (TCD) versus Syn.
P < .01 Allo (+T cells) versus Allo (TCD).
P < .01 Allo (+T cells) versus Syn.