Apoptosis of CLL Patients' Leukemic Cells After Treatment With 10 μmol/L Rolipram, 40 μmol/L forskolin, or 100 μmol/L dbcAMP
| Patient No. . | Rai . | Drug Res. . | Basal . | Roli . | Fsk . | R/Fs . | dbcAMP . |
|---|---|---|---|---|---|---|---|
| 1 | III | — | 47 | 80 | ND | ND | ND |
| 2 | IV | Ch, Cy | 18 ± 2 | 79 | 46 | 89 | ND |
| 3 | IV | Ch, CH | 38 | 77 ± 1 | 59 ± 0 | 79 ± 1 | 71 ± 3 |
| 4 | I | — | 31 ± 2 | 76 ± 2 | ND | ND | ND |
| 5 | IV | — | 18 | 57 ± 2 | ND | ND | ND |
| 6 | IV | Ch | 17 ± 4 | 54 ± 1 | 30 ± 4 | 60 ± 2 | 39 ± 4 |
| 7 | II | Ch | 13 ± 2 | 47 ± 2 | 32 ± 0 | 58 ± 0 | 32 ± 4 |
| 8 | 0 | — | 5 | 46 | 22 | 55-150 | ND |
| 9 | IV | Ch, Cy, F1 | 4 ± 0 | 44 ± 3 | 17 ± 4 | 77 ± 2 | 46 ± 1 |
| 10 | I | — | 11 | 44 ± 0 | 26 ± 4 | 56 ± 0 | ND |
| 11 | 0 | — | 24 ± 4 | 40 ± 0 | 40 ± 3 | 69 ± 0 | 39 ± 2 |
| 12 | IV | Ch, F1, 2C | 0 ± 4 | 38 ± 1 | 43 ± 1 | 68 ± 0 | ND |
| 13 | 0 | — | 2 ± 0 | 34 ± 0 | ND | ND | 23 ± 6 |
| 14-151 | I | — | 13 ± 0 | 14 ± 0 | 19 ± 3 | 37 ± 3 | 22 ± 0 |
| Patient No. . | Rai . | Drug Res. . | Basal . | Roli . | Fsk . | R/Fs . | dbcAMP . |
|---|---|---|---|---|---|---|---|
| 1 | III | — | 47 | 80 | ND | ND | ND |
| 2 | IV | Ch, Cy | 18 ± 2 | 79 | 46 | 89 | ND |
| 3 | IV | Ch, CH | 38 | 77 ± 1 | 59 ± 0 | 79 ± 1 | 71 ± 3 |
| 4 | I | — | 31 ± 2 | 76 ± 2 | ND | ND | ND |
| 5 | IV | — | 18 | 57 ± 2 | ND | ND | ND |
| 6 | IV | Ch | 17 ± 4 | 54 ± 1 | 30 ± 4 | 60 ± 2 | 39 ± 4 |
| 7 | II | Ch | 13 ± 2 | 47 ± 2 | 32 ± 0 | 58 ± 0 | 32 ± 4 |
| 8 | 0 | — | 5 | 46 | 22 | 55-150 | ND |
| 9 | IV | Ch, Cy, F1 | 4 ± 0 | 44 ± 3 | 17 ± 4 | 77 ± 2 | 46 ± 1 |
| 10 | I | — | 11 | 44 ± 0 | 26 ± 4 | 56 ± 0 | ND |
| 11 | 0 | — | 24 ± 4 | 40 ± 0 | 40 ± 3 | 69 ± 0 | 39 ± 2 |
| 12 | IV | Ch, F1, 2C | 0 ± 4 | 38 ± 1 | 43 ± 1 | 68 ± 0 | ND |
| 13 | 0 | — | 2 ± 0 | 34 ± 0 | ND | ND | 23 ± 6 |
| 14-151 | I | — | 13 ± 0 | 14 ± 0 | 19 ± 3 | 37 ± 3 | 22 ± 0 |
Cultures were performed for 3 days and the percentage of apoptotic cells measured by Hoechst 33342 flow cytometry. SEMs are shown for samples done in triplicate; the remainder of the values are the mean of duplicate cultures.
Abbreviations: Res., resistance; Ch, chlorambucil; CH, CHOP; Cy, cyclophosphamide; Fl, fludarabine; 2C, 2-chlorodeoxyadenosine.
Rolipram was at 1 μmol/L.
This patient had a significant population of cells with prolymphocyte morphology.