Effect of EGTA and Three Different PKC Inhibitors on Adhesive and Migratory Ability of Aspirin (ASA)-Treated T Cells
| . | Adherent T Cells (%) . | Migrated T Cells (%) . |
|---|---|---|
| Basal (medium) | 28.9 ± 5 | 7.0 ± 2 |
| ASA (300 μg/mL) | 17.3 ± 33-150 | 3.3 ± 13-150 |
| ASA + EGTA (1 mmol/L) | 26.9 ± 43-151 | 6.8 ± 33-151 |
| ASA + staurosporin (1 μmol/L) | 20.0 ± 43-152 | 4.9 ± 23-152 |
| ASA + calphostin C (1 μmol/L) | 17.1 ± 33-150 | 4.8 ± 23-152 |
| ASA + H-7 (100 μmol/L) | 16.1 ± 33-150 | 2.9 ± 13-150 |
| . | Adherent T Cells (%) . | Migrated T Cells (%) . |
|---|---|---|
| Basal (medium) | 28.9 ± 5 | 7.0 ± 2 |
| ASA (300 μg/mL) | 17.3 ± 33-150 | 3.3 ± 13-150 |
| ASA + EGTA (1 mmol/L) | 26.9 ± 43-151 | 6.8 ± 33-151 |
| ASA + staurosporin (1 μmol/L) | 20.0 ± 43-152 | 4.9 ± 23-152 |
| ASA + calphostin C (1 μmol/L) | 17.1 ± 33-150 | 4.8 ± 23-152 |
| ASA + H-7 (100 μmol/L) | 16.1 ± 33-150 | 2.9 ± 13-150 |
Data are expressed as the mean ± SEM of six separate experiments. T cells were incubated with PKC inhibitors or EGTA for 30 minutes at 37°C, washed, and then tested in the adhesion system. Preincubation of T cells with staurosporin, calphostin C, or EGTA alone did not affect adhesion and migration of T cells, whereas H-7 slightly, but significantly, reduced T-cell adhesion (22.8 ± 4%;P < .05 v basal). Numbers significantly different from the basal values are in bold.
P < .01.
P < .01 versus ASA alone.
P < .05.