Expression Induced by Activated-HUVEC of the β2 Integrin Activation Reporter Epitope Recognized by the MoAb 24: Effect of T-Cell Preincubation With 300 μg/mL ASA for 30 Minutes
| . | 24 Epitope Expression (MFI) . | |
|---|---|---|
| Medium . | ASA Preincubation . | |
| Unstimulated T cells | 14 ± 4 | 16 ± 7 |
| HUVEC-nonadherent T cells | 18 ± 6 | 17 ± 6 |
| HUVEC-adherent T cells | 86 ± 18 | 34 ± 7* |
| HUVEC-migrated T cells | 168 ± 31 | 80 ± 22* |
| . | 24 Epitope Expression (MFI) . | |
|---|---|---|
| Medium . | ASA Preincubation . | |
| Unstimulated T cells | 14 ± 4 | 16 ± 7 |
| HUVEC-nonadherent T cells | 18 ± 6 | 17 ± 6 |
| HUVEC-adherent T cells | 86 ± 18 | 34 ± 7* |
| HUVEC-migrated T cells | 168 ± 31 | 80 ± 22* |
Data are expressed as the mean ± SEM of six separate experiments. Numbers significantly different from the values of the resting T-cell population are in bold (P < .001). A positive control for the induction of 24 epitope was performed by incubating unstimulated T cells with 500 μmol/L Mn2+, as previously described.46 In this experimental condition, the MFI increased from 14 ± 4 to 79 ± 18 (P < .001).
P < .001 versus medium.