Primary Antibodies Used in the Immunohistochemical Analyses, Together With Optimum Dilutions and Microwave Pretreatment Times
| Antigen . | Antibody Species and Clone . | Source . | Optimum Dilution . | Microwave Time (min) . |
|---|---|---|---|---|
| LMP1 | Mouse, CS1-4 | Young et al,66 Dako Ltd. Cat No. M0897 | 1:500 | 20 |
| LMP2 | Rat, 14B7-1-1 | Gift from E. Kremmer, Munich, Germany22 | ||
| TAP-1 | Mouse, Ring4 | Gift from Dr J. Trowsdale, Oxford | 1:1,000 | 0 |
| TAP-2 | Mouse, AK-7 | Gift from Dr J. Trowsdale, Oxford | 1:500 | 0 |
| HLA A2.1 | Mouse, MA2.1 | Gift from Dr S. Lee, University of Birmingham | 1:50 | 0 |
| HLA I | Mouse, HC-10 | Gift from Dr P. Stern | 1:50 | 20 |
| Antigen . | Antibody Species and Clone . | Source . | Optimum Dilution . | Microwave Time (min) . |
|---|---|---|---|---|
| LMP1 | Mouse, CS1-4 | Young et al,66 Dako Ltd. Cat No. M0897 | 1:500 | 20 |
| LMP2 | Rat, 14B7-1-1 | Gift from E. Kremmer, Munich, Germany22 | ||
| TAP-1 | Mouse, Ring4 | Gift from Dr J. Trowsdale, Oxford | 1:1,000 | 0 |
| TAP-2 | Mouse, AK-7 | Gift from Dr J. Trowsdale, Oxford | 1:500 | 0 |
| HLA A2.1 | Mouse, MA2.1 | Gift from Dr S. Lee, University of Birmingham | 1:50 | 0 |
| HLA I | Mouse, HC-10 | Gift from Dr P. Stern | 1:50 | 20 |