Granulocyte Apoptosis After 12 Hours of Serum Starvation in Patients With PNH and Controls
| Source of Granulocytes . | No. of Samples . | % Apoptosis (mean ± 1 SD) . | P Value . |
|---|---|---|---|
| Normal controls | 20 | 55.0 ± 12.0 | |
| PNH patients | 26 | 38.8 ± 14.1 | <.001 |
| Aplastic anemia | |||
| Yes | 9 | 37.6 ± 17.5 | |
| No | 17 | 39.2 ± 12.5 | .79 |
| Neutrophil count | |||
| ≤2,000/μL | 8 | 40.7 ± 17.9 | |
| 2,000-4,000/μL | 10 | 38.9 ± 11.5 | .92 |
| ≥4,000/μL | 8 | 37.7 ± 15.3 | |
| % GPI deficiency | |||
| ≤40% | 5 | 43.8 ± 18.6 | |
| 40%-90% | 6 | 33.4 ± 18.2 | .49 |
| ≥90% | 12 | 37.2 ± 9.3 |
| Source of Granulocytes . | No. of Samples . | % Apoptosis (mean ± 1 SD) . | P Value . |
|---|---|---|---|
| Normal controls | 20 | 55.0 ± 12.0 | |
| PNH patients | 26 | 38.8 ± 14.1 | <.001 |
| Aplastic anemia | |||
| Yes | 9 | 37.6 ± 17.5 | |
| No | 17 | 39.2 ± 12.5 | .79 |
| Neutrophil count | |||
| ≤2,000/μL | 8 | 40.7 ± 17.9 | |
| 2,000-4,000/μL | 10 | 38.9 ± 11.5 | .92 |
| ≥4,000/μL | 8 | 37.7 ± 15.3 | |
| % GPI deficiency | |||
| ≤40% | 5 | 43.8 ± 18.6 | |
| 40%-90% | 6 | 33.4 ± 18.2 | .49 |
| ≥90% | 12 | 37.2 ± 9.3 |
Stratification of PNH patients according to prior aplastic anemia, degree of neutropenia, or clonal dominance showed no statistical differences in rates of apoptosis. Three patients did not have simultaneous quantitation of GPI-deficient granulocytes.