Results of Absorption Study of Group 1 Sera With Untransfected L, L αβ and L βIX Cells
| After Absorbing With: . | Binding of Quinine-Dependent Antibodies to: . | |
|---|---|---|
| L αβ . | L βIX . | |
| L0 Cells | + | + |
| L αβ Cells | − | + |
| L βIX Cells | + | − |
| After Absorbing With: . | Binding of Quinine-Dependent Antibodies to: . | |
|---|---|---|
| L αβ . | L βIX . | |
| L0 Cells | + | + |
| L αβ Cells | − | + |
| L βIX Cells | + | − |
L0 cells are untransfected L cells. L αβ cells are stably transfected with GPIbα cDNA and GPIbβ cDNA and have been confirmed to express the corresponding proteins. L βIX cells are stably transfected with GPIbβ and GPIX cDNA and are expressing GPIbβ and GPIX proteins. Group 1 patient sera were absorbed with L0 cells, L αβ cells, and L βIX cells and then assayed for quinine-dependent antibody binding to L αβ or L βIX cells.