Ability of MIP-1α to Inhibit the Proliferation of Primitive Normal Progenitors in LTC Adherent Layers Can Be Neutralized by the Simultaneous Addition of Anti–MIP-1α Antibodies
| Treatment . | % Kill After3H-Thymidine . | |
|---|---|---|
| Primitive BFU-E . | Primitive CFU-GM . | |
| None | 44 ± 6 | 43 ± 3 |
| MIP-1α | 2 ± 4 | 3 ± 2 |
| MIP-1α + anti–MIP-1α | 37 ± 7 | 42 ± 5 |
| MIP-1α + control Ig | 0 ± 6 | 1 ± 6 |
| Treatment . | % Kill After3H-Thymidine . | |
|---|---|---|
| Primitive BFU-E . | Primitive CFU-GM . | |
| None | 44 ± 6 | 43 ± 3 |
| MIP-1α | 2 ± 4 | 3 ± 2 |
| MIP-1α + anti–MIP-1α | 37 ± 7 | 42 ± 5 |
| MIP-1α + control Ig | 0 ± 6 | 1 ± 6 |
MIP-1α at 100 ng/mL, anti–MIP-1α and control Ig at 100 μg/mL. Results shown are the mean ±SEM of data obtained in six independent experiments. The effect of anti–MIP-1α is highly significant by comparison with cultures to which only MIP-1α was added (P < .005), whereas the effect of control Ig is not (P > .3).