Induction of the Murine Macrophage 12-Lipoxygenase by Selected Cytokines
| No. . | Mice . | Cytokine . | n . | 12/15-HETE Formation-150 (μg/106 cells) . |
|---|---|---|---|---|
| 1 | NMRI | no (control) | 6 | 2.13 ± 0.25 |
| IL-4 | 3 | 3.67 ± 0.30 | ||
| IL-10 | 3 | 2.18 ± 0.18 | ||
| 2 | STAT6(+/+) controls | no (control) | 4 | 11.3 ± 1.7 |
| IL-4 | 4 | 18.7 ± 1.9 | ||
| IL-13 | 4 | 17.8 ± 0.7 | ||
| STAT6(−/−) | no (control) | 4 | 10.2 ± 2.3 | |
| IL-4 | 4 | 5.5 ± 1.5 | ||
| IL-13 | 4 | 3.0 ± 0.2 |
| No. . | Mice . | Cytokine . | n . | 12/15-HETE Formation-150 (μg/106 cells) . |
|---|---|---|---|---|
| 1 | NMRI | no (control) | 6 | 2.13 ± 0.25 |
| IL-4 | 3 | 3.67 ± 0.30 | ||
| IL-10 | 3 | 2.18 ± 0.18 | ||
| 2 | STAT6(+/+) controls | no (control) | 4 | 11.3 ± 1.7 |
| IL-4 | 4 | 18.7 ± 1.9 | ||
| IL-13 | 4 | 17.8 ± 0.7 | ||
| STAT6(−/−) | no (control) | 4 | 10.2 ± 2.3 | |
| IL-4 | 4 | 5.5 ± 1.5 | ||
| IL-13 | 4 | 3.0 ± 0.2 |
Murine peritoneal macrophages were prepared from normal mice (experiment 1), from homozygous STAT6 deficient animals (experiment 2), and from inbred control mice (experiment 2) by peritoneal lavage and adherence to plastic dishes (see Materials and Methods). The cells were cultured in the presence or absence of cytokines (1 nmol/L) at 37°C, scraped from the dishes after 4 days, and sonicated in the presence arachidonic acid. After incubation for 15 minutes at 37°C, the lipids were extracted and the lipoxygenase products were quantified by RP-HPLC (see Materials and Methods). For experiment 1, male NMRI mice were used, and a final arachidonic acid concentration of 30 μmol/L was adjusted. For experiment 2 STAT6-deficient mice and the corresponding inbred controls were used. Here the arachidonic acid concentration was 0.1 mmol/L.
Mean ± SD.