Effect of RANKL on cytokine and chemokine mRNA expression in H/RS cell lines
| Cell line . | Fold increase in cytokine mRNA expression . | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| IFN-γ . | IL-8 . | RANTES . | CCR-4 . | IL-9 . | IL-9Rα . | IL-15 . | IL-15Rα . | IL-13 . | IL-13Rα . | |
| HDLM-2 | 2 | — | 1.5 | 3 | 3 | — | 2 | — | 2 | — |
| HD-MYZ | — | 7 | — | — | — | 4.6 | — | 9 | — | 3 |
| L-428 | 4 | — | 3 | — | — | — | — | — | 3 | — |
| KMH-2 | — | — | — | — | — | — | 2 | — | — | — |
| Cell line . | Fold increase in cytokine mRNA expression . | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| IFN-γ . | IL-8 . | RANTES . | CCR-4 . | IL-9 . | IL-9Rα . | IL-15 . | IL-15Rα . | IL-13 . | IL-13Rα . | |
| HDLM-2 | 2 | — | 1.5 | 3 | 3 | — | 2 | — | 2 | — |
| HD-MYZ | — | 7 | — | — | — | 4.6 | — | 9 | — | 3 |
| L-428 | 4 | — | 3 | — | — | — | — | — | 3 | — |
| KMH-2 | — | — | — | — | — | — | 2 | — | — | — |
Cells were incubated with RPMI or RANKL (1 μg/mL) for 6 to 24 hours before mRNA levels were determined using the RNAse protection assay.