Analysis of VH gene sequences
| Case no. . | Primer combination . | Corresponding VH germline . | In frame . | Percentage mutation . | Observed mutations in FR . | R/S . | P . | No. clones sequenced . | Ongoing mutation . | ||
|---|---|---|---|---|---|---|---|---|---|---|---|
| R . | S . | Observed . | Expected . | ||||||||
| 1 | FR1 | V3-21 | + | 7.4 | 7 | 5 | 1.4 | 2.8 | .0327* | 7 | − |
| 2 | FR1 | V3-48 | + | 5.6 | 4 | 3 | 1.3 | 2.9 | .0156* | 8 | − |
| 3 | FR1 | V4-30.4 | − | 18.8 | NA‡ | NA‡ | NA‡ | NA‡ | NA‡ | 8 | − |
| 4 | FR1 | V3-09 | + | 6.3 | 8 | 5 | 1.6 | 3.0 | .1434 | 7 | − |
| 5 | FR1 | V5-a | + | 14.4 | 17 | 5 | 3.4 | 3.3 | .0252* | 8 | − |
| 6 | FR1 | V3-33 | + | 6.7 | 7 | 3 | 2.3 | 2.9 | .0651 | 9 | − |
| 7 | FR1 | V1-69 | + | 9.3 | 7 | 7 | 1.0 | 3.0 | .0026* | 6 | − |
| 8a | FR1 | V4-39 | + | 16.9 | 14 | 14 | 1.0 | 2.6 | .0007* | 8 | − |
| 8b | FR1 | V4-39 | + | 16.9 | 14 | 14 | 1.0 | 2.6 | .0007* | 7 | + |
| 9† | FR2 | V4-34 | + | 14.9 | 8 | 12 | 0.7 | 2.8 | .0069* | 3 | − |
| 10 | FR2 | V2-05 | + | 10.5 | 4 | 7 | 0.6 | 3.2 | .0088* | 6 | − |
| 11 | FR2 | V3-30 | + | 30.9 | 21 | 12 | 1.8 | 3.2 | .0396* | 5 | − |
| 12† | FR2 | V1-02 | + | 10.5 | 9 | 10 | 0.9 | 3.4 | .0339* | 3 | − |
| 13a | FR2 | V3-20 | + | 17.3 | 8 | 7 | 1.1 | 3.2 | .0033* | 5 | − |
| 13b | FR2 | V3-20 | + | 17.3 | 8 | 7 | 1.1 | 3.2 | .0033 | 3 | − |
| Case no. . | Primer combination . | Corresponding VH germline . | In frame . | Percentage mutation . | Observed mutations in FR . | R/S . | P . | No. clones sequenced . | Ongoing mutation . | ||
|---|---|---|---|---|---|---|---|---|---|---|---|
| R . | S . | Observed . | Expected . | ||||||||
| 1 | FR1 | V3-21 | + | 7.4 | 7 | 5 | 1.4 | 2.8 | .0327* | 7 | − |
| 2 | FR1 | V3-48 | + | 5.6 | 4 | 3 | 1.3 | 2.9 | .0156* | 8 | − |
| 3 | FR1 | V4-30.4 | − | 18.8 | NA‡ | NA‡ | NA‡ | NA‡ | NA‡ | 8 | − |
| 4 | FR1 | V3-09 | + | 6.3 | 8 | 5 | 1.6 | 3.0 | .1434 | 7 | − |
| 5 | FR1 | V5-a | + | 14.4 | 17 | 5 | 3.4 | 3.3 | .0252* | 8 | − |
| 6 | FR1 | V3-33 | + | 6.7 | 7 | 3 | 2.3 | 2.9 | .0651 | 9 | − |
| 7 | FR1 | V1-69 | + | 9.3 | 7 | 7 | 1.0 | 3.0 | .0026* | 6 | − |
| 8a | FR1 | V4-39 | + | 16.9 | 14 | 14 | 1.0 | 2.6 | .0007* | 8 | − |
| 8b | FR1 | V4-39 | + | 16.9 | 14 | 14 | 1.0 | 2.6 | .0007* | 7 | + |
| 9† | FR2 | V4-34 | + | 14.9 | 8 | 12 | 0.7 | 2.8 | .0069* | 3 | − |
| 10 | FR2 | V2-05 | + | 10.5 | 4 | 7 | 0.6 | 3.2 | .0088* | 6 | − |
| 11 | FR2 | V3-30 | + | 30.9 | 21 | 12 | 1.8 | 3.2 | .0396* | 5 | − |
| 12† | FR2 | V1-02 | + | 10.5 | 9 | 10 | 0.9 | 3.4 | .0339* | 3 | − |
| 13a | FR2 | V3-20 | + | 17.3 | 8 | 7 | 1.1 | 3.2 | .0033* | 5 | − |
| 13b | FR2 | V3-20 | + | 17.3 | 8 | 7 | 1.1 | 3.2 | .0033 | 3 | − |
Sequences have been submitted to the EMBL/GenBank/DDBJ database under accession numbers AJ292059 through AJ292070. See Table 1 footnote for descriptions of cases 8b and 13b.
FR indicates frame region; R, replacement mutation; S, silent mutation; NA, not analyzed.
P < .05.
No intraclonal diversity was detectable, but this was of limited significance owing to low number of sequenced clones.
Not analyzed because of nonfunctional rearrangement.