Increase in Hb Oxidation Rate Caused by Iron Chelates
| Experimental Condition . | n . | Increase in Hb Oxidation Rate (μmol/L/min) . | |
|---|---|---|---|
| HbA . | HbS . | ||
| Hb + Fe3+ + L1 | 4 | .025 ± .008 | .026 ± .003 |
| Hb + Fe3+ + NTA | 8 | .825 ± .045 | .850 ± .047 |
| Hb + Fe3+ + EDTA | 9 | .130 ± .007 | .138 ± .014 |
| Hb + Fe3+ + citrate | 9 | .026 ± .005 | .031 ± .012 |
| Hb + Fe3+ + ATP | 8 | .349 ± .028 | .341 ± .029 |
| Hb + Fe3+ + ADP | 8 | .340 ± .018 | .340 ± .024 |
| Hb + Fe3+ + 23DPG | 3 | .121 ± .025 | .096 ± .010 |
| Hb + Fe3+ + GSH | 3 | .016 ± .006 | .010 ± .011 |
| Experimental Condition . | n . | Increase in Hb Oxidation Rate (μmol/L/min) . | |
|---|---|---|---|
| HbA . | HbS . | ||
| Hb + Fe3+ + L1 | 4 | .025 ± .008 | .026 ± .003 |
| Hb + Fe3+ + NTA | 8 | .825 ± .045 | .850 ± .047 |
| Hb + Fe3+ + EDTA | 9 | .130 ± .007 | .138 ± .014 |
| Hb + Fe3+ + citrate | 9 | .026 ± .005 | .031 ± .012 |
| Hb + Fe3+ + ATP | 8 | .349 ± .028 | .341 ± .029 |
| Hb + Fe3+ + ADP | 8 | .340 ± .018 | .340 ± .024 |
| Hb + Fe3+ + 23DPG | 3 | .121 ± .025 | .096 ± .010 |
| Hb + Fe3+ + GSH | 3 | .016 ± .006 | .010 ± .011 |
Conditions were as follows: same as for Table 1, except for the addition of 50 μmol/L Fe3+ in the form of a chelate. Results (mean ± SD) are shown as the absolute increase in Hb oxidation rate over that measured in presence of Hb plus chelator, but without added iron. For this set of experiments, these basal rates of oxidation were .029 ± .002 for HbA and .042 ± .003 for HbS. Chelator to iron ratios were 1:1 for EDTA, 2:1 for NTA, 4:1 for GSH, 10:1 for ATP and 23DPG, 20:1 for citrate and ADP, and 40:1 for L1. All oxidation rate increments were significant; but for any given chelator, there was no significant difference for HbA v HbS.