Effect of Cytokine-Mediated Priming of FMLP-Stimulated H2O2 Production in Patients With SCD Compared With Healthy Controls
| Stimulus . | H2O2 Production . | |||
|---|---|---|---|---|
| SteadyState . | Control . | Crisis . | Control . | |
| FCS + PBS | ||||
| % positive | 5 | 5 | 5 | 5 |
| MCF (absolute value) | 278 ± 48 | 225 ± 31 | 446 ± 99 | 348 ± 69 |
| % MCF | 100 | 100 | 100 | 100 |
| FCS + FMLP | ||||
| % positive | 20 ± 4 | 17 ± 2 | 10 ± 1 | 17 ± 3 |
| MCF (absolute value) | 326 ± 65 | 258 ± 34 | 421 ± 109 | 289 ± 70 |
| % MCF | 100 | 100 | 100 | 100 |
| GM-CSF + PBS | ||||
| % positive | 8 ± 1 | 9 ± 1 | 10 ± 2 | 13 ± 1 |
| % MCF | 104 ± 4 | 110 ± 6 | 107 ± 7 | 106 ± 8 |
| GM-CSF + FMLP | ||||
| % positive | 75 ± 2‡ | 90 ± 3 | 45 ± 1‡ | 87 ± 7 |
| % MCF | 173 ± 20 | 239 ± 26 | 147 ± 18* | 279 ± 46 |
| TNF-α + PBS | ||||
| % positive | 7 ± 1* | 19 ± 5 | 8 ± 2* | 12 ± 1 |
| % MCF | 99 ± 4 | 102 ± 6 | 99 ± 5 | 109 ± 8 |
| TNF-α + FMLP | ||||
| % positive | 70 ± 6† | 88 ± 4 | 36 ± 3‡ | 84 ± 5 |
| % MCF | 151 ± 17* | 234 ± 34 | 136 ± 17† | 240 ± 40 |
| Stimulus . | H2O2 Production . | |||
|---|---|---|---|---|
| SteadyState . | Control . | Crisis . | Control . | |
| FCS + PBS | ||||
| % positive | 5 | 5 | 5 | 5 |
| MCF (absolute value) | 278 ± 48 | 225 ± 31 | 446 ± 99 | 348 ± 69 |
| % MCF | 100 | 100 | 100 | 100 |
| FCS + FMLP | ||||
| % positive | 20 ± 4 | 17 ± 2 | 10 ± 1 | 17 ± 3 |
| MCF (absolute value) | 326 ± 65 | 258 ± 34 | 421 ± 109 | 289 ± 70 |
| % MCF | 100 | 100 | 100 | 100 |
| GM-CSF + PBS | ||||
| % positive | 8 ± 1 | 9 ± 1 | 10 ± 2 | 13 ± 1 |
| % MCF | 104 ± 4 | 110 ± 6 | 107 ± 7 | 106 ± 8 |
| GM-CSF + FMLP | ||||
| % positive | 75 ± 2‡ | 90 ± 3 | 45 ± 1‡ | 87 ± 7 |
| % MCF | 173 ± 20 | 239 ± 26 | 147 ± 18* | 279 ± 46 |
| TNF-α + PBS | ||||
| % positive | 7 ± 1* | 19 ± 5 | 8 ± 2* | 12 ± 1 |
| % MCF | 99 ± 4 | 102 ± 6 | 99 ± 5 | 109 ± 8 |
| TNF-α + FMLP | ||||
| % positive | 70 ± 6† | 88 ± 4 | 36 ± 3‡ | 84 ± 5 |
| % MCF | 151 ± 17* | 234 ± 34 | 136 ± 17† | 240 ± 40 |
Data shown are the mean ± 1 SE of 7 experiments (GM-CSF) and 9 experiments (TNF-α) with SCD patients in steady-state, and 6 experiments with SCD patients in crisis. The percentage of positive responding cells stimulated with PBS was set at 5%. The MCF of growth factor–treated cells ± FMLP stimulation was expressed as a percentage of the MCF of their respective FCS diluent controls (the absolute values in arbitrary fluorescence units for these are shown). Statistical significance of the difference between patient and controls was tested by the Wilcoxon matched-pairs signed-ranks test.
.05 > P > .01.
.01 > P > .001.
P < .001.